EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.184 | expression in Escherichia coli | Kluyveromyces marxianus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.184 | 0.028 | - |
NADPH | pH 6.0, 25°C | Kluyveromyces marxianus | |
1.1.1.184 | 0.034 | - |
NADPH | pH 6.0, 25°C | Kluyveromyces marxianus | |
1.1.1.184 | 0.43 | - |
methyl glyoxal | pH 6.0, 25°C | Kluyveromyces marxianus | |
1.1.1.184 | 1.6 | - |
methyl glyoxal | pH 6.0, 25°C | Kluyveromyces marxianus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.184 | 35800 | - |
gel filtration | Kluyveromyces marxianus |
1.1.1.184 | 36900 | - |
gel filtration | Kluyveromyces marxianus |
1.1.1.184 | 37000 | - |
1 * 37000, SDS-PAGE | Kluyveromyces marxianus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.184 | Kluyveromyces marxianus | A0A0E4AX59 | - |
- |
1.1.1.184 | Kluyveromyces marxianus | A0A0E4AY21 | - |
- |
1.1.1.184 | Kluyveromyces marxianus DMB1 | A0A0E4AX59 | - |
- |
1.1.1.184 | Kluyveromyces marxianus DMB1 | A0A0E4AY21 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.184 | 0.58 | - |
substrate benzaldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 0.6 | - |
substrate benzaldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 0.65 | - |
substrate furfural, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 0.96 | - |
substrate methyl glyoxal, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 1.1 | - |
substrate p-anisaldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 1.2 | - |
substrate furfural, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 1.5 | - |
substrate hexanal, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 2 | - |
substrate hexanal, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 2.1 | - |
substrate octanal, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 2.1 | - |
substrate valeraldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 3 | - |
substrate heptanal, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 3.2 | - |
substrate methyl glyoxal, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 3.3 | - |
substrate isovaleraldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 4.2 | - |
substrate isovaleraldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 4.5 | - |
substrate p-anisaldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 5.3 | - |
substrate octanal, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 5.9 | - |
substrate valeraldehyde, pH 6.0, 25°C | Kluyveromyces marxianus |
1.1.1.184 | 6.9 | - |
substrate heptanal, pH 6.0, 25°C | Kluyveromyces marxianus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.184 | benzaldehyde + NADPH + H+ | - |
Kluyveromyces marxianus | benzyl alcohol + NADP+ | - |
? | |
1.1.1.184 | benzaldehyde + NADPH + H+ | - |
Kluyveromyces marxianus DMB1 | benzyl alcohol + NADP+ | - |
? | |
1.1.1.184 | furfural + NADPH + H+ | - |
Kluyveromyces marxianus | furfuryl alcohol + NADP+ | - |
? | |
1.1.1.184 | heptanal + NADPH + H+ | - |
Kluyveromyces marxianus | heptan-1-ol + NADP+ | - |
? | |
1.1.1.184 | heptanal + NADPH + H+ | - |
Kluyveromyces marxianus DMB1 | heptan-1-ol + NADP+ | - |
? | |
1.1.1.184 | hexanal + NADPH + H+ | - |
Kluyveromyces marxianus | hexan-1-ol + NADP+ | - |
? | |
1.1.1.184 | isovaleraldehyde + NADPH + H+ | - |
Kluyveromyces marxianus | isoamyl alcohol + NADP+ | - |
? | |
1.1.1.184 | isovaleraldehyde + NADPH + H+ | - |
Kluyveromyces marxianus DMB1 | isoamyl alcohol + NADP+ | - |
? | |
1.1.1.184 | methyl glyoxal + NADPH + H+ | - |
Kluyveromyces marxianus | ? + NADP+ | - |
? | |
1.1.1.184 | methyl glyoxal + NADPH + H+ | - |
Kluyveromyces marxianus DMB1 | ? + NADP+ | - |
? | |
1.1.1.184 | additional information | strong activities are observed toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward 5-hydroxymethylfurfural, propionaldehyde, D-alanine, L-alanine,D-lactate, L-lactate or pyruvate. Enzyme does not exhibit NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol | Kluyveromyces marxianus | ? | - |
? | |
1.1.1.184 | additional information | strong activities are observed toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine,D-lactate, L-lactate or pyruvate. Enzyme does not exhibit NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol | Kluyveromyces marxianus | ? | - |
? | |
1.1.1.184 | additional information | strong activities are observed toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward 5-hydroxymethylfurfural, propionaldehyde, D-alanine, L-alanine,D-lactate, L-lactate or pyruvate. Enzyme does not exhibit NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol | Kluyveromyces marxianus DMB1 | ? | - |
? | |
1.1.1.184 | additional information | strong activities are observed toward linear aldehydes, such as 1-heptanal, valeraldehyde and 1-octanal, but no activity toward HMF, propionaldehyde, D-alanine, L-alanine,D-lactate, L-lactate or pyruvate. Enzyme does not exhibit NADP+-dependent oxidative activity toward corresponding alcohol analogs, including 1-hexanol, 1-heptanol, isoamyl alcohol, isobutanol, 1-octanol and 2-propanol | Kluyveromyces marxianus DMB1 | ? | - |
? | |
1.1.1.184 | octanal + NADPH + H+ | - |
Kluyveromyces marxianus | octan-1-ol + NADP+ | - |
? | |
1.1.1.184 | p-anisaldehyde + NADPH + H+ | - |
Kluyveromyces marxianus | p-anisalcohol + NADP+ | - |
? | |
1.1.1.184 | valeraldehyde + NADPH + H+ | - |
Kluyveromyces marxianus | pentan-1-ol + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.184 | monomer | 1 * 37000, SDS-PAGE | Kluyveromyces marxianus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.184 | YGL039w1 | - |
Kluyveromyces marxianus |
1.1.1.184 | YGL039w2 | - |
Kluyveromyces marxianus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.184 | 45 | 50 | - |
Kluyveromyces marxianus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.184 | 45 | - |
20 min, loss of more than 50% of activity | Kluyveromyces marxianus |
1.1.1.184 | 47 | - |
melting temperature | Kluyveromyces marxianus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.184 | 2.5 | - |
methyl glyoxal | pH 6.0, 25°C | Kluyveromyces marxianus | |
1.1.1.184 | 2.67 | - |
NADPH | pH 6.0, 25°C | Kluyveromyces marxianus | |
1.1.1.184 | 3.67 | - |
NADPH | pH 6.0, 25°C | Kluyveromyces marxianus | |
1.1.1.184 | 4 | - |
methyl glyoxal | pH 6.0, 25°C | Kluyveromyces marxianus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.184 | 6 | - |
- |
Kluyveromyces marxianus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.184 | additional information | no activity with NADH | Kluyveromyces marxianus | |
1.1.1.184 | NADPH | exclusive use of NADPH | Kluyveromyces marxianus |