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Literature summary extracted from

  • Wagschal, K.; Jordan, D.B.; Lee, C.C.; Younger, A.; Braker, J.D.; Chan, V.J.
    Biochemical characterization of uronate dehydrogenases from three pseudomonads, Chromohalobacter salixigens, and Polaromonas naphthalenivorans (2015), Enzyme Microb. Technol., 69, 62-68.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.203 gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas fluorescens
1.1.1.203 gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas mendocina
1.1.1.203 gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Chromohalobacter salexigens
1.1.1.203 gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Polaromonas naphthalenivorans
1.1.1.203 gene udh, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas syringae pv. tomato

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.203 enzyme structure, PDB ID 3AY3 Chromohalobacter salexigens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.203 D-galactaro-1,5-lactone noncompetitve versus NAD+ and beta-D-glucuronate Polaromonas naphthalenivorans
1.1.1.203 additional information inhibition mechanisms, overview Polaromonas naphthalenivorans
1.1.1.203 NADH competitve versus NAD+, noncompetitive versus beta-D-glucuronate Polaromonas naphthalenivorans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.203 additional information
-
 additional information Michaelis-Menten kinetics and thermodynamics Pseudomonas fluorescens
1.1.1.203 additional information
-
 additional information Michaelis-Menten kinetics and thermodynamics Pseudomonas mendocina
1.1.1.203 additional information
-
 additional information Michaelis-Menten kinetics and thermodynamics Pseudomonas syringae pv. tomato
1.1.1.203 additional information
-
 additional information steady-state and stopped-flow kinetics, Michaelis-Menten kinetics, and thermodynamics Polaromonas naphthalenivorans
1.1.1.203 0.053
-
 beta-D-galacturonate pH 8.0, 25°C, method A Pseudomonas mendocina
1.1.1.203 0.06
-
 NAD+ pH 8.0, 25°C, method B Polaromonas naphthalenivorans
1.1.1.203 0.078
-
 NAD+ pH 8.0, 25°C, method B Pseudomonas mendocina
1.1.1.203 0.106
-
 beta-D-galacturonate pH 8.0, 25°C Pseudomonas fluorescens
1.1.1.203 0.107
-
 beta-D-galacturonate pH 8.0, 25°C Pseudomonas syringae pv. tomato
1.1.1.203 0.138
-
 beta-D-galacturonate pH 8.0, 25°C, method B Pseudomonas mendocina
1.1.1.203 0.148
-
 beta-D-galacturonate pH 8.0, 25°C, method A Polaromonas naphthalenivorans
1.1.1.203 0.16
-
 beta-D-galacturonate pH 8.0, 25°C Chromohalobacter salexigens
1.1.1.203 0.24
-
 NAD+ pH 8.0, 25°C Chromohalobacter salexigens
1.1.1.203 0.25
-
 NAD+ pH 8.0, 25°C Pseudomonas fluorescens
1.1.1.203 0.25
-
 NAD+ pH 8.0, 25°C, method A Pseudomonas mendocina
1.1.1.203 0.279
-
 beta-D-galacturonate pH 8.0, 25°C, method B Polaromonas naphthalenivorans
1.1.1.203 0.37
-
 NAD+ pH 8.0, 25°C Pseudomonas syringae pv. tomato
1.1.1.203 0.4
-
 NAD+ pH 8.0, 25°C, method A Polaromonas naphthalenivorans
1.1.1.203 0.48
-
 beta-D-glucuronate pH 8.0, 25°C, method A Pseudomonas mendocina
1.1.1.203 0.69
-
 beta-D-glucuronate pH 8.0, 25°C Pseudomonas fluorescens
1.1.1.203 1.08
-
 beta-D-glucuronate pH 8.0, 25°C, method B Pseudomonas mendocina
1.1.1.203 1.27
-
 beta-D-glucuronate pH 8.0, 25°C Chromohalobacter salexigens
1.1.1.203 1.3
-
 beta-D-glucuronate pH 8.0, 25°C, method A Polaromonas naphthalenivorans
1.1.1.203 1.34
-
 beta-D-glucuronate pH 8.0, 25°C Pseudomonas syringae pv. tomato
1.1.1.203 1.94
-
 beta-D-glucuronate pH 8.0, 25°C, method B Polaromonas naphthalenivorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.203 beta-D-galacturonate + NAD+ Pseudomonas fluorescens
-
 D-galactaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-galacturonate + NAD+ Pseudomonas mendocina
-
 D-galactaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-galacturonate + NAD+ Chromohalobacter salexigens
-
 D-galactaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-galacturonate + NAD+ Polaromonas naphthalenivorans
-
 D-galactaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-galacturonate + NAD+ Pseudomonas syringae pv. tomato
-
 D-galactaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-galacturonate + NAD+ Pseudomonas fluorescens Pf0-1
-
 D-galactaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-galacturonate + NAD+ Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000
-
 D-galactaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-glucuronate + NAD+ Pseudomonas fluorescens
-
 D-glucaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-glucuronate + NAD+ Pseudomonas mendocina
-
 D-glucaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-glucuronate + NAD+ Chromohalobacter salexigens
-
 D-glucaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-glucuronate + NAD+ Polaromonas naphthalenivorans
-
 D-glucaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-glucuronate + NAD+ Pseudomonas syringae pv. tomato
-
 D-glucaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-glucuronate + NAD+ Pseudomonas fluorescens Pf0-1
-
 D-glucaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-glucuronate + NAD+ Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000
-
 D-glucaro-1,5-lactone + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.203 Chromohalobacter salexigens
-
-
-
1.1.1.203 Polaromonas naphthalenivorans
-
-
-
1.1.1.203 Pseudomonas fluorescens
-
-
-
1.1.1.203 Pseudomonas fluorescens Pf0-1
-
-
-
1.1.1.203 Pseudomonas mendocina
-
-
-
1.1.1.203 Pseudomonas syringae pv. tomato Q888H1
-
-
1.1.1.203 Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000 Q888H1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.203 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Pseudomonas fluorescens
1.1.1.203 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Pseudomonas mendocina
1.1.1.203 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Chromohalobacter salexigens
1.1.1.203 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Polaromonas naphthalenivorans
1.1.1.203 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Pseudomonas syringae pv. tomato

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.203 beta-D-galacturonate + NAD+
-
 Pseudomonas fluorescens D-galactaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-galacturonate + NAD+
-
 Pseudomonas mendocina D-galactaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-galacturonate + NAD+
-
 Chromohalobacter salexigens D-galactaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-galacturonate + NAD+
-
 Polaromonas naphthalenivorans D-galactaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-galacturonate + NAD+
-
 Pseudomonas syringae pv. tomato D-galactaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-galacturonate + NAD+
-
 Pseudomonas fluorescens Pf0-1 D-galactaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-galacturonate + NAD+
-
 Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000 D-galactaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-glucuronate + NAD+
-
 Pseudomonas fluorescens D-glucaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-glucuronate + NAD+
-
 Pseudomonas mendocina D-glucaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-glucuronate + NAD+
-
 Chromohalobacter salexigens D-glucaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-glucuronate + NAD+
-
 Polaromonas naphthalenivorans D-glucaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-glucuronate + NAD+
-
 Pseudomonas syringae pv. tomato D-glucaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-glucuronate + NAD+
-
 Pseudomonas fluorescens Pf0-1 D-glucaro-1,5-lactone + NADH + H+
-
 ?
1.1.1.203 beta-D-glucuronate + NAD+
-
 Pseudomonas syringae pv. tomato ATCC BAA-871 / DC3000 D-glucaro-1,5-lactone + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.203 More amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview Pseudomonas fluorescens
1.1.1.203 More amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview Pseudomonas mendocina
1.1.1.203 More amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview Chromohalobacter salexigens
1.1.1.203 More amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview Polaromonas naphthalenivorans
1.1.1.203 More amino acid sequence comparisons with Rossmann fold, NAD+ and substrate binding sequences, and catalytic residues TyrxxxLys and S/T, overview Pseudomonas syringae pv. tomato

Synonyms

EC Number Synonyms Comment Organism
1.1.1.203 CsUDH
-
 Chromohalobacter salexigens
1.1.1.203 PfUDH
-
 Pseudomonas fluorescens
1.1.1.203 PmUDH
-
 Pseudomonas mendocina
1.1.1.203 PnUDH
-
 Polaromonas naphthalenivorans
1.1.1.203 PSPTO_1053
-
 Pseudomonas syringae pv. tomato
1.1.1.203 PsUDH
-
 Pseudomonas syringae pv. tomato
1.1.1.203 Udh
-
 Pseudomonas fluorescens
1.1.1.203 Udh
-
 Pseudomonas mendocina
1.1.1.203 Udh
-
 Chromohalobacter salexigens
1.1.1.203 Udh
-
 Polaromonas naphthalenivorans
1.1.1.203 Udh
-
 Pseudomonas syringae pv. tomato

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.203 25
-
 assay at Pseudomonas fluorescens
1.1.1.203 25
-
 assay at Pseudomonas mendocina
1.1.1.203 25
-
 assay at Chromohalobacter salexigens
1.1.1.203 25
-
 assay at Polaromonas naphthalenivorans
1.1.1.203 25
-
 assay at Pseudomonas syringae pv. tomato

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.203 15.3
-
 beta-D-galacturonate pH 8.0, 25°C Chromohalobacter salexigens
1.1.1.203 17.1
-
 beta-D-galacturonate pH 8.0, 25°C Pseudomonas fluorescens
1.1.1.203 17.4
-
 beta-D-galacturonate pH 8.0, 25°C, method A Pseudomonas mendocina
1.1.1.203 17.9
-
 beta-D-galacturonate pH 8.0, 25°C, method B Pseudomonas mendocina
1.1.1.203 27.8
-
 beta-D-galacturonate pH 8.0, 25°C Pseudomonas syringae pv. tomato
1.1.1.203 33.8
-
 beta-D-galacturonate pH 8.0, 25°C, method A Polaromonas naphthalenivorans
1.1.1.203 51.6
-
 beta-D-galacturonate pH 8.0, 25°C, method B Polaromonas naphthalenivorans
1.1.1.203 101
-
 beta-D-glucuronate pH 8.0, 25°C, method B Pseudomonas mendocina
1.1.1.203 119
-
 beta-D-glucuronate pH 8.0, 25°C Chromohalobacter salexigens
1.1.1.203 152
-
 beta-D-glucuronate pH 8.0, 25°C Pseudomonas fluorescens
1.1.1.203 159
-
 beta-D-glucuronate pH 8.0, 25°C, method A Pseudomonas mendocina
1.1.1.203 212
-
 beta-D-glucuronate pH 8.0, 25°C, method B Polaromonas naphthalenivorans
1.1.1.203 217
-
 beta-D-glucuronate pH 8.0, 25°C Pseudomonas syringae pv. tomato
1.1.1.203 274
-
 beta-D-glucuronate pH 8.0, 25°C, method A Polaromonas naphthalenivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.203 8
-
 assay at Pseudomonas fluorescens
1.1.1.203 8
-
 assay at Pseudomonas mendocina
1.1.1.203 8
-
 assay at Chromohalobacter salexigens
1.1.1.203 8
-
 assay at Polaromonas naphthalenivorans
1.1.1.203 8
-
 assay at Pseudomonas syringae pv. tomato

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.203 NAD+
-
 Pseudomonas fluorescens
1.1.1.203 NAD+
-
 Pseudomonas mendocina
1.1.1.203 NAD+
-
 Chromohalobacter salexigens
1.1.1.203 NAD+
-
 Pseudomonas syringae pv. tomato
1.1.1.203 NAD+ binding kinetics, overview Polaromonas naphthalenivorans
1.1.1.203 NADH
-
 Pseudomonas fluorescens
1.1.1.203 NADH
-
 Pseudomonas mendocina
1.1.1.203 NADH
-
 Chromohalobacter salexigens
1.1.1.203 NADH
-
 Polaromonas naphthalenivorans
1.1.1.203 NADH
-
 Pseudomonas syringae pv. tomato

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.203 additional information
-
 additional information inhibition kinetics, overview Polaromonas naphthalenivorans

General Information

EC Number General Information Comment Organism
1.1.1.203 evolution UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site Pseudomonas fluorescens
1.1.1.203 evolution UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site Pseudomonas mendocina
1.1.1.203 evolution UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site Chromohalobacter salexigens
1.1.1.203 evolution UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site Polaromonas naphthalenivorans
1.1.1.203 evolution UDH is a member of the short-chain dehydrogenase/reductase(SDR) superfamily, whose members typically contain a single domain having a highly variable C-terminal substrate binding site, reflecting the diverse nature of SDR substrates and attendant substrate binding site structures, and a conserved N-terminal cofactor binding site Pseudomonas syringae pv. tomato

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.203 additional information
-
 additional information Michaelis-Menten kinetics and thermodynamics Chromohalobacter salexigens
1.1.1.203 93.5
-
 beta-D-glucuronate pH 8.0, 25°C, method B Pseudomonas mendocina
1.1.1.203 93.7
-
 beta-D-glucuronate pH 8.0, 25°C Chromohalobacter salexigens
1.1.1.203 96
-
 beta-D-galacturonate pH 8.0, 25°C Chromohalobacter salexigens
1.1.1.203 109
-
 beta-D-glucuronate pH 8.0, 25°C, method B Polaromonas naphthalenivorans
1.1.1.203 130
-
 beta-D-galacturonate pH 8.0, 25°C, method B Pseudomonas mendocina
1.1.1.203 161
-
 beta-D-galacturonate pH 8.0, 25°C Pseudomonas fluorescens
1.1.1.203 162
-
 beta-D-glucuronate pH 8.0, 25°C Pseudomonas syringae pv. tomato
1.1.1.203 185
-
 beta-D-galacturonate pH 8.0, 25°C, method B Polaromonas naphthalenivorans
1.1.1.203 211
-
 beta-D-glucuronate pH 8.0, 25°C, method A Polaromonas naphthalenivorans
1.1.1.203 220
-
 beta-D-glucuronate pH 8.0, 25°C Pseudomonas fluorescens
1.1.1.203 228
-
 beta-D-galacturonate pH 8.0, 25°C, method A Polaromonas naphthalenivorans
1.1.1.203 260
-
 beta-D-galacturonate pH 8.0, 25°C Pseudomonas syringae pv. tomato
1.1.1.203 328
-
 beta-D-galacturonate pH 8.0, 25°C, method A Pseudomonas mendocina
1.1.1.203 331
-
 beta-D-glucuronate pH 8.0, 25°C, method A Pseudomonas mendocina