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Literature summary extracted from

  • Yamashita, Y.; Ferri, S.; Huynh, M.L.; Shimizu, H.; Yamaoka, H.; Sode, K.
    Direct electron transfer type disposable sensor strip for glucose sensing employing an engineered FAD glucose dehydrogenase (2013), Enzyme Microb. Technol., 52, 123-128.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.9 expressed in Escherichia coli BL21 cells Burkholderia cepacia

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.9 S326C the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S326E the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S326G the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S326H the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S326K the mutant shows increased activity toward D-glucose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S326Q the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S326Q/S365Y the mutant is virtually non-reactive to maltose while retaining high D-glucose dehydrogenase activity Burkholderia cepacia
1.1.5.9 S326R the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S326T the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S326V the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S326Y the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365A the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365C the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365D the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365E the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365F the mutant shows reduced activity toward D-glucose and increased activity with maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365G the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365H the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365I the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365K the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365L the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365M the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365N the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365P the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365Q the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365R the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365T the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365V the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365W the mutant shows reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365Y the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365Y the mutant shows strongly reduced activity toward D-glucose and maltose compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365Y/S326E the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365Y/S326G the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365Y/S326H the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365Y/S326K the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365Y/S326Q the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365Y/S326R the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365Y/S326T the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia
1.1.5.9 S365Y/S326V the mutant shows reduced activity compared to the wild type enzyme Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.9 Burkholderia cepacia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.9 DEAE-5PW column chromatography and Superdex-200 gel filtration Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol
-
 Burkholderia cepacia D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 maltose + 2,6-dichlorophenolindophenol
-
 Burkholderia cepacia maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.9 FAD glucose dehydrogenase
-
 Burkholderia cepacia
1.1.5.9 FADGDH
-
 Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.9 FAD
-
 Burkholderia cepacia