EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.28 | synthesis | D-LDH is a candidate for thermophilic D-lactic acid production | Thermodesulfatator indicus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.28 | gene WP_013906894 , recombinant expression of the enzyme in Bacillus licheniformis strain ATCC 14580 MW3 derivative BL2 with a deleted native gene ldh, encoding L-LDH. Gene WP_013906894 is introduced to redirect the carbon flux, the genes for 2,3-butanediol production, alsS and alsD, are replaced with WP_013906894, giving strains BN11, BN22, BN44, and BN66 | Thermodesulfatator indicus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.28 | additional information | fermentation conditions are critical for microbial growth and lactic acid secretion, optimization of enzyme and D-lactic acid production by the enzyme from Thermodesulfatator indicus in Bacillus licheniformis strains B11 and BA11, overview. Disruption of the lactate permease LctP in host strains BN11 and BA11 results in reductions in D-lactic acid (28.6 and 35.2%, respectively). Replacement of LctP inhost strain BN11 with the mesophilic permease LldP of Escherichia coli also leads to a 12.1% decrease in D-lactic acid accumulation | Thermodesulfatator indicus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.28 | Co2+ | activates | Thermodesulfatator indicus | |
1.1.1.28 | Zn2+ | activates | Thermodesulfatator indicus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.28 | pyruvate + NADH + H+ | Thermodesulfatator indicus | - |
(R)-lactate + NAD+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.28 | Thermodesulfatator indicus | F8A9V0 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 30.2 | - |
pyruvate reduction, pH 6.0, 70°C | Thermodesulfatator indicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.28 | glyoxylate + NADH + H+ | - |
Thermodesulfatator indicus | hydroxyacetate + NAD+ | - |
r | |
1.1.1.28 | additional information | no activity towards L-lactate | Thermodesulfatator indicus | ? | - |
? | |
1.1.1.28 | oxaloacetate + NADH + H+ | - |
Thermodesulfatator indicus | D-malate + NAD+ | - |
r | |
1.1.1.28 | phenylpyruvate + NADH + H+ | - |
Thermodesulfatator indicus | D-phenyllactate + NAD+ | - |
r | |
1.1.1.28 | pyruvate + NADH + H+ | - |
Thermodesulfatator indicus | (R)-lactate + NAD+ | - |
? | |
1.1.1.28 | pyruvate + NADH + H+ | preferred subtrate | Thermodesulfatator indicus | (R)-lactate + NAD+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.28 | tetramer | - |
Thermodesulfatator indicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.28 | D-isomer specific 2-hydroxyacid dehydrogenase NAD-binding protein | - |
Thermodesulfatator indicus |
1.1.1.28 | D-LDH | - |
Thermodesulfatator indicus |
1.1.1.28 | D-LDH-like enzyme | - |
Thermodesulfatator indicus |
1.1.1.28 | LdhTi | - |
Thermodesulfatator indicus |
1.1.1.28 | WP_013906894 | - |
Thermodesulfatator indicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 70 | - |
- |
Thermodesulfatator indicus |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 30 | 100 | activity range, high activity at 60-80°C | Thermodesulfatator indicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 6 | - |
- |
Thermodesulfatator indicus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 3 | 11 | activity range | Thermodesulfatator indicus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.28 | NADH | - |
Thermodesulfatator indicus |