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Literature summary extracted from

  • Chen, C.; Liu, B.; Xu, Y.; Utkina, N.; Zhou, D.; Danilov, L.; Torgov, V.; Veselovsky, V.; Feng, L.
    Biochemical characterization of the novel alpha-1, 3-galactosyltransferase WclR from Escherichia coli O3 (2016), Carbohydr. Res., 430, 36-43.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.87 expression in Escherichia coli Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.87 Fe2+ assay in presence of 5 mM Mg2+, Mn2+, or Fe2+ Escherichia coli
2.4.1.87 Mg2+ assay in presence of 5 mM Mg2+, Mn2+, or Fe2+ Escherichia coli
2.4.1.87 Mn2+ assay in presence of 5 mM Mg2+, Mn2+, or Fe2+ Escherichia coli
2.4.1.343 Fe2+ Mg2+, Mn2+ or Fe2+ is required Escherichia coli
2.4.1.343 Mg2+ assay in presence of 5 mM Mg2+. Mg2+, Mn2+ or Fe2+ is required Escherichia coli
2.4.1.343 Mg2+ Mg2+, Mn2+ or Fe2+ is required Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.343 43948
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.343 UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Escherichia coli
-
 UDP + alpha-D-Gal-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.343 UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Escherichia coli O3
-
 UDP + alpha-D-Gal-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.87 Escherichia coli B5T072
-
-
2.4.1.87 Escherichia coli O3 B5T072
-
-
2.4.1.343 Escherichia coli B5T072
-
-
2.4.1.343 Escherichia coli O3 B5T072
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.87 additional information no donors: UDP-Glc, UDP-GlcNAc, UDPGalNAc, CMP-Neu and GDP-Man Escherichia coli ?
-
 ?
2.4.1.87 additional information no donors: UDP-Glc, UDP-GlcNAc, UDPGalNAc, CMP-Neu and GDP-Man Escherichia coli O3 ?
-
 ?
2.4.1.87 UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosamine-diphospho-O-(CH2)9CH3
-
 Escherichia coli UDP + alpha-D-galactosyl-(1->3)-N-acetyl-alpha-D-glucosamine-diphospho-O-(CH2)9CH3
-
 ?
2.4.1.87 UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosamine-diphospho-O-(CH2)9CH3
-
 Escherichia coli O3 UDP + alpha-D-galactosyl-(1->3)-N-acetyl-alpha-D-glucosamine-diphospho-O-(CH2)9CH3
-
 ?
2.4.1.343 additional information the diphosphate and lipid moieties may be critical for acceptor recognition by the enzyme. No substrates: alpha-D-glucosyl-diphosphodecanol, N-acetyl-alpha-D-glucosaminyl-diphospho-1,11-undecadiol 11-phenyl ether, N-acetyl-alpha-D-glucosamine Escherichia coli ?
-
 ?
2.4.1.343 additional information the diphosphate and lipid moieties may be critical for acceptor recognition by the enzyme. No substrates: alpha-D-glucosyl-diphosphodecanol, N-acetyl-alpha-D-glucosaminyl-diphospho-1,11-undecadiol 11-phenyl ether, N-acetyl-alpha-D-glucosamine Escherichia coli O3 ?
-
 ?
2.4.1.343 UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Escherichia coli UDP + alpha-D-Gal-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.343 UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Escherichia coli UDP + alpha-D-Gal-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol the galactose residue is linked to the GlcNAc via an alpha-1,3 linkage ?
2.4.1.343 UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Escherichia coli O3 UDP + alpha-D-Gal-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.4.1.343 UDP-alpha-D-galactose + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Escherichia coli O3 UDP + alpha-D-Gal-(1->3)-alpha-D-GlcNAc-diphospho-ditrans,octacis-undecaprenol the galactose residue is linked to the GlcNAc via an alpha-1,3 linkage ?

Subunits

EC Number Subunits Comment Organism
2.4.1.87 ? x * 70240, calculated from sequence, x * 66000, SDS-PAGE Escherichia coli
2.4.1.343 ? x * 70240, recombinant GST-fusion protein, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.4.1.87 wclR
-
 Escherichia coli
2.4.1.343 wclR
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.87 5 9
-
 Escherichia coli