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Literature summary extracted from
- Novel fungal FAD glucose dehydrogenase derived from Aspergillus niger for glucose enzyme sensor strips (2017), Biosens. Bioelectron., 87, 305-311.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.5.9 | expressed in Escherichia coli BL21 (DE3) cells | Aspergillus niger |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.5.9 | 3.8 | - |
D-glucose | at pH 6.5, temperature not specified in the publication | Aspergillus niger |  |
| 1.1.5.9 | 22 | - |
D-xylose | at pH 6.5, temperature not specified in the publication | Aspergillus niger | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.5.9 | 60000 | - |
x * 60000, SDS-PAGE | Aspergillus niger |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.5.9 | Aspergillus niger | - |
- |
- |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 1.1.5.9 | using 0.05 mM FAD,10% (v/v) glycerol,and 20 mM potassium phosphate buffer, pH 6.5 | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.5.9 | D-glucose + 2,6-dichlorophenolindophenol | - |
Aspergillus niger | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.5.9 | D-xylose + 2,6-dichlorophenolindophenol | 1.8% activity compared to D-glucose | Aspergillus niger | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.5.9 | additional information | less than 1% activity with maltose, cellobiose, lactose, mannose, fructose, allose, galactose, and sucrose | Aspergillus niger | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.5.9 | ? | x * 60000, SDS-PAGE | Aspergillus niger |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.5.9 | FAD glucose dehydrogenase | - |
Aspergillus niger |
| 1.1.5.9 | GDH | - |
Aspergillus niger |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.5.9 | FAD | - |
Aspergillus niger | |
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Release 2023.1 (January 2023)
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