BRENDA - Enzyme Database

Membrane-bound glycerol dehydrogenase catalyzes oxidation of D-pentonates to 4-dehydro-D-pentonates, D-fructose to 5-dehydro-D-fructose, and D-psicose to 5-dehydro-D-psicose

Ano, Y.; Hours, R.A.; Akakabe, Y.; Kataoka, N.; Yakushi, T.; Matsushita, K.; Adachi, O.; Biosci. Biotechnol. Biochem. 81, 411-418 (2017)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.99.22
EDTA
-
Gluconobacter thailandicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.99.22
membrane
-
Gluconobacter thailandicus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.99.22
Ca2+
activates
Gluconobacter thailandicus
1.1.99.22
additional information
Mg2+, Sr2+, Ba2+, and several kinds of lantanides do not activate the enzyme
Gluconobacter thailandicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.99.22
80000
-
x * 80000, SDS-PAGE
Gluconobacter thailandicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.99.22
Gluconobacter thailandicus
-
-
-
1.1.99.22
Gluconobacter thailandicus NBRC 3258
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.99.22
CM-Toyopearl C-650 column chromatography, and Sephadex G-200 gel filtration
Gluconobacter thailandicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.99.22
D-arabitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-arabitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-arabonate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
4-dehydro-D-arabonate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-arabonate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
4-dehydro-D-arabonate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-erythronate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
3-dehydro-D-erythronate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-erythronate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
3-dehydro-D-erythronate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-fructose + oxidized 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-fructose + oxidized 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-psicose + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
5-dehydro-D-psicose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-psicose + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
5-dehydro-D-psicose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-ribitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-ribonate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
4-dehydro-D-ribonate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-sorbitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
glycerol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
glycerone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
glycerol + phenazine methosulfate
-
740188
Gluconobacter thailandicus
glycerone + reduced phenazine methosulfate
-
-
-
?
1.1.99.22
glycerol + potassium ferricyanide
-
740188
Gluconobacter thailandicus
glycerone + potassium ferrocyanide
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.99.22
?
x * 80000, SDS-PAGE
Gluconobacter thailandicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.99.22
pyrroloquinoline quinone
-
Gluconobacter thailandicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.99.22
pyrroloquinoline quinone
-
Gluconobacter thailandicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.99.22
EDTA
-
Gluconobacter thailandicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.99.22
membrane
-
Gluconobacter thailandicus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.99.22
Ca2+
activates
Gluconobacter thailandicus
1.1.99.22
additional information
Mg2+, Sr2+, Ba2+, and several kinds of lantanides do not activate the enzyme
Gluconobacter thailandicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.99.22
80000
-
x * 80000, SDS-PAGE
Gluconobacter thailandicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.99.22
CM-Toyopearl C-650 column chromatography, and Sephadex G-200 gel filtration
Gluconobacter thailandicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.99.22
D-arabitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-arabitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-arabonate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
4-dehydro-D-arabonate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-arabonate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
4-dehydro-D-arabonate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-erythronate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
3-dehydro-D-erythronate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-erythronate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
3-dehydro-D-erythronate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-fructose + oxidized 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-fructose + oxidized 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
5-dehydro-D-fructose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-psicose + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
5-dehydro-D-psicose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-psicose + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus NBRC 3258
5-dehydro-D-psicose + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-ribitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-ribonate + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
4-dehydro-D-ribonate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
D-sorbitol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
? + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
glycerol + 2,6-dichlorophenolindophenol
-
740188
Gluconobacter thailandicus
glycerone + reduced 2,6-dichlorophenolindophenol
-
-
-
?
1.1.99.22
glycerol + phenazine methosulfate
-
740188
Gluconobacter thailandicus
glycerone + reduced phenazine methosulfate
-
-
-
?
1.1.99.22
glycerol + potassium ferricyanide
-
740188
Gluconobacter thailandicus
glycerone + potassium ferrocyanide
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.99.22
?
x * 80000, SDS-PAGE
Gluconobacter thailandicus