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Literature summary extracted from

  • Takeda, K.; Ishida, T.; Igarashi, K.; Samejima, M.; Nakamura, N.; Ohno, H.
    Effect of amines as activators on the alcohol-oxidizing activity of pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase (2014), Biosci. Biotechnol. Biochem., 78, 1195-1198.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.5.5 ammonia
-
 Pseudomonas putida
1.1.5.5 Butylamine
-
 Pseudomonas putida
1.1.5.5 Dimethylamine
-
 Pseudomonas putida
1.1.5.5 dipropylamine
-
 Pseudomonas putida
1.1.5.5 ethylamine
-
 Pseudomonas putida
1.1.5.5 heptylamine
-
 Pseudomonas putida
1.1.5.5 Hexylamine second best activating amine Pseudomonas putida
1.1.5.5 Isobutylamine
-
 Pseudomonas putida
1.1.5.5 methylamine
-
 Pseudomonas putida
1.1.5.5 octylamine
-
 Pseudomonas putida
1.1.5.5 pentylamine best activating amine Pseudomonas putida
1.1.5.5 Propylamine
-
 Pseudomonas putida
1.1.5.5 tert-Butylamine
-
 Pseudomonas putida
1.1.5.5 triethylamine
-
 Pseudomonas putida
1.1.5.5 trimethylamine
-
 Pseudomonas putida
1.1.5.5 tripropylamine
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.5 0.14
-
 ethanol in the presence of pentylamine, at pH 9.0 and 25°C Pseudomonas putida
1.1.5.5 0.21
-
 ethanol in the presence of ethylamine, at pH 9.0 and 25°C Pseudomonas putida
1.1.5.5 0.35
-
 1-propanol in the presence of pentylamine, at pH 9.0 and 25°C Pseudomonas putida
1.1.5.5 0.43
-
 1-propanol in the presence of ethylamine, at pH 9.0 and 25°C Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.5 Pseudomonas putida
-
-
-
1.1.5.5 Pseudomonas putida KT 2240
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.5 1-propanol + 2,6-dichlorophenolindophenol
-
 Pseudomonas putida propionaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.5 1-propanol + 2,6-dichlorophenolindophenol
-
 Pseudomonas putida KT 2240 propionaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.5 ethanol + 2,6-dichlorophenolindophenol
-
 Pseudomonas putida acetaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.5 ethanol + 2,6-dichlorophenolindophenol
-
 Pseudomonas putida KT 2240 acetaldehyde + reduced 2,6-dichlorophenolindophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.5.5 PQQ-ADH
-
 Pseudomonas putida
1.1.5.5 pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenase
-
 Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.5.5 24.9
-
 ethanol in the presence of ethylamine, at pH 9.0 and 25°C Pseudomonas putida
1.1.5.5 32.6
-
 1-propanol in the presence of ethylamine, at pH 9.0 and 25°C Pseudomonas putida
1.1.5.5 56.6
-
 ethanol in the presence of pentylamine, at pH 9.0 and 25°C Pseudomonas putida
1.1.5.5 73.1
-
 1-propanol in the presence of pentylamine, at pH 9.0 and 25°C Pseudomonas putida

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.5.5 73
-
 1-propanol in the presence of ethylamine, at pH 9.0 and 25°C Pseudomonas putida
1.1.5.5 115
-
 ethanol in the presence of ethylamine, at pH 9.0 and 25°C Pseudomonas putida
1.1.5.5 209
-
 1-propanol in the presence of pentylamine, at pH 9.0 and 25°C Pseudomonas putida
1.1.5.5 395
-
 ethanol in the presence of pentylamine, at pH 9.0 and 25°C Pseudomonas putida