Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shiota, M.; Yamazaki, T.; Yoshimatsu, K.; Kojima, K.; Tsugawa, W.; Ferri, S.; Sode, K.
    An Fe-S cluster in the conserved Cys-rich region in the catalytic subunit of FAD-dependent dehydrogenase complexes (2016), Bioelectrochemistry, 112, 178-183.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.9 C212S inactive Burkholderia cepacia
1.1.5.9 C213S the mutant shows 68% of wild type activity Burkholderia cepacia
1.1.5.9 C218S inactive Burkholderia cepacia
1.1.5.9 C222S inactive Burkholderia cepacia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.9 0.97
-
D-glucose wild type enzyme, at pH 7.0, temperature not specified in the publication Burkholderia cepacia
1.1.5.9 0.99
-
D-glucose mutant enzyme C213S, at pH 7.0, temperature not specified in the publication Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.9 60000
-
-
Burkholderia cepacia

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.9 Burkholderia cepacia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.9 HisTrap nickel affinity chromatography Burkholderia cepacia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol
-
Burkholderia cepacia D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
?

Subunits

EC Number Subunits Comment Organism
1.1.5.9 ? x * 59832, catalytic subunit, calculated from amino acid sequence Burkholderia cepacia

Synonyms

EC Number Synonyms Comment Organism
1.1.5.9 FAD dependent glucose dehydrogenase
-
Burkholderia cepacia
1.1.5.9 FADGDH
-
Burkholderia cepacia

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.9 FAD
-
Burkholderia cepacia
1.1.5.9 [3Fe-4S]-center
-
Burkholderia cepacia