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Literature summary extracted from

  • Salvi, F.; Rodriguez, I.; Hamelberg, D.; Gadda, G.
    Role of F357 as an oxygen gate in the oxidative half-reaction of choline oxidase (2016), Biochemistry, 55, 1473-1484.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.17 expressed in Escherichia coli Rosetta(DE3)pLysS cells Arthrobacter globiformis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.17 F357A the mutant shows about 240fold reduced catalytic efficiency compared to the wild type enzyme Arthrobacter globiformis
1.1.3.17 M62A the mutant shows about 3fold reduced catalytic efficiency compared to the wild type enzyme Arthrobacter globiformis
1.1.3.17 M62A/F357A the enzyme shows a lack of the isomerization detected in wild type choline oxidase, and a lack of saturation with an oxygen concentration as high as 1 mM, while most other kinetic parameters are similar to those of wild type choline oxidase Arthrobacter globiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.17 0.4
-
choline wild type enzyme, at pH 7.0 and 25°C Arthrobacter globiformis
1.1.3.17 0.8
-
choline mutant enzyme M62A, at pH 7.0 and 25°C Arthrobacter globiformis
1.1.3.17 9
-
choline mutant enzyme M62A/F357A, at pH 7.0 and 25°C Arthrobacter globiformis
1.1.3.17 12
-
choline mutant enzyme F357A, at pH 7.0 and 25°C Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.17 betaine aldehyde + O2 + H2O Arthrobacter globiformis
-
betaine + H2O2
-
?
1.1.3.17 choline + O2 Arthrobacter globiformis
-
betaine aldehyde + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.17 Arthrobacter globiformis Q7X2H8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.17 betaine aldehyde + O2 + H2O
-
Arthrobacter globiformis betaine + H2O2
-
?
1.1.3.17 choline + O2
-
Arthrobacter globiformis betaine aldehyde + H2O2
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.17 choline:oxygen 1-reductase
-
Arthrobacter globiformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.17 2.5
-
choline mutant enzyme F357A, at pH 7.0 and 25°C Arthrobacter globiformis
1.1.3.17 6.8
-
choline mutant enzyme M62A/F357A, at pH 7.0 and 25°C Arthrobacter globiformis
1.1.3.17 12.5
-
choline mutant enzyme M62A, at pH 7.0 and 25°C Arthrobacter globiformis
1.1.3.17 20
-
choline wild type enzyme, at pH 7.0 and 25°C Arthrobacter globiformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.17 FAD
-
Arthrobacter globiformis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.17 0.21
-
choline mutant enzyme F357A, at pH 7.0 and 25°C Arthrobacter globiformis
1.1.3.17 0.8
-
choline mutant enzyme M62A/F357A, at pH 7.0 and 25°C Arthrobacter globiformis
1.1.3.17 16
-
choline mutant enzyme M62A, at pH 7.0 and 25°C Arthrobacter globiformis
1.1.3.17 50
-
choline wild type enzyme, at pH 7.0 and 25°C Arthrobacter globiformis