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Literature summary extracted from

  • Smitherman, C.; Rungsrisuriyachai, K.; Germann, M.W.; Gadda, G.
    Identification of the catalytic base for alcohol activation in choline oxidase (2015), Biochemistry, 54, 413-421.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.17 expressed in Escherichia coli Rosetta(DE3)pLysS cells Arthrobacter globiformis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.17 H351Q the kcat and kcat/Km values of the H351Q emutant in atmospheric oxygen are 45 and 5000fold lower than those of the wild type enzyme, respectively Arthrobacter globiformis
1.1.3.17 H466Q inactive Arthrobacter globiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.17 0.6
-
 O2 wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 25°C Arthrobacter globiformis
1.1.3.17 67
-
 O2 mutant enzyme H351Q, in 50 mM potassium phosphate (pH 7.0), at 25°C Arthrobacter globiformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.17 betaine aldehyde + O2 + H2O Arthrobacter globiformis
-
 glycine betaine + H2O2
-
 ?
1.1.3.17 choline + O2 Arthrobacter globiformis
-
 betaine aldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.17 Arthrobacter globiformis Q7X2H8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.17 betaine aldehyde + O2 + H2O
-
 Arthrobacter globiformis glycine betaine + H2O2
-
 ?
1.1.3.17 choline + O2
-
 Arthrobacter globiformis betaine aldehyde + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.17 CHO
-
 Arthrobacter globiformis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.17 0.33
-
 O2 mutant enzyme H351Q, in 50 mM potassium phosphate (pH 7.0), at 25°C Arthrobacter globiformis
1.1.3.17 15
-
 O2 wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 25°C Arthrobacter globiformis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.17 FAD
-
 Arthrobacter globiformis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.17 0.0049
-
 O2 mutant enzyme H351Q, in 50 mM potassium phosphate (pH 7.0), at 25°C Arthrobacter globiformis
1.1.3.17 25
-
 O2 wild type enzyme, in 50 mM potassium phosphate (pH 7.0), at 25°C Arthrobacter globiformis