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Literature summary extracted from

  • Elsen, N.L.; Bailey, L.J.; Hauser, A.D.; Fox, B.G.
    Role for threonine 201 in the catalytic cycle of the soluble diiron hydroxylase toluene 4-monooxygenase (2009), Biochemistry, 48, 3838-3846.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.236
-
 Pseudomonas mendocina

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.13.236 comparison of X-ray crystal structures of resting and reduced mutant T201A in complex with effector T4moD with structures of wild-type reveals changes in the positions of several key active site residues Pseudomonas mendocina

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.236 T201A mutation has no impact on steady-state catalysis or coupling. Mutant T201A gives stoichometric release of H2O2 during reaction in the absence of substrate and has a faster first-order rate constant for product formation than wild-type Pseudomonas mendocina
1.14.13.236 T201G mutation has no impact on steady-state catalysis or coupling Pseudomonas mendocina
1.14.13.236 T201S mutation has no impact on steady-state catalysis or coupling Pseudomonas mendocina

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.236 Pseudomonas mendocina Q00456 subunit TmoA
-
1.14.13.236 Pseudomonas mendocina KR1 Q00456 subunit TmoA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.236 anisole + NADH + H+ + O2
-
 Pseudomonas mendocina 4-methoxyphenol + NAD+ + H2O 87% efficiency ?
1.14.13.236 anisole + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 4-methoxyphenol + NAD+ + H2O 87% efficiency ?
1.14.13.236 benzene + NADH + H+ + O2
-
 Pseudomonas mendocina phenol + NAD+ + H2O 98% efficiency ?
1.14.13.236 benzene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 phenol + NAD+ + H2O 98% efficiency ?
1.14.13.236 chlorobenzene + NADH + H+ + O2
-
 Pseudomonas mendocina 4-chlorophenol + NAD+ + H2O 39% efficiency ?
1.14.13.236 chlorobenzene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 4-chlorophenol + NAD+ + H2O 39% efficiency ?
1.14.13.236 nitrobenzene + NADH + H+ + O2
-
 Pseudomonas mendocina 4-nitrophenol + NAD+ + H2O 12% efficiency ?
1.14.13.236 nitrobenzene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 4-nitrophenol + NAD+ + H2O 12% efficiency ?
1.14.13.236 toluene + NADH + H+ + O2
-
 Pseudomonas mendocina 4-methylphenol + NAD+ + H2O 194% efficiency ?
1.14.13.236 toluene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 4-methylphenol + NAD+ + H2O 194% efficiency ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.236 0.013
-
 nitrobenzene wild-type, pH 7.5, 4°C Pseudomonas mendocina
1.14.13.236 0.034
-
 nitrobenzene mutant T201A, pH 7.5, 4°C Pseudomonas mendocina
1.14.13.236 0.075
-
 chlorobenzene wild-type, pH 7.5, 4°C Pseudomonas mendocina
1.14.13.236 0.087
-
 chlorobenzene mutant T201A, pH 7.5, 4°C Pseudomonas mendocina
1.14.13.236 0.1
-
 Benzene wild-type, pH 7.5, 4°C Pseudomonas mendocina
1.14.13.236 0.16
-
 anisole wild-type, pH 7.5, 4°C Pseudomonas mendocina
1.14.13.236 0.21
-
 Toluene wild-type, pH 7.5, 4°C Pseudomonas mendocina
1.14.13.236 0.23
-
 Toluene mutant T201A, pH 7.5, 4°C Pseudomonas mendocina
1.14.13.236 0.23
-
 anisole mutant T201A, pH 7.5, 4°C Pseudomonas mendocina
1.14.13.236 0.31
-
 Benzene mutant T201A, pH 7.5, 4°C Pseudomonas mendocina

General Information

EC Number General Information Comment Organism
1.14.13.236 physiological function during reactions in the absence of substrate, wild-type toluene 4-monooxygenase hydroxylase T4moH slowly consumes O2 but only a negligible amount of H2O2 is released Pseudomonas mendocina