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Literature summary extracted from

  • Moe, L.A.; Hu, Z.; Deng, D.; Austin, R.N.; Groves, J.T.; Fox, B.G.
    Remarkable aliphatic hydroxylation by the diiron enzyme toluene 4-monooxygenase in reactions with radical or cation diagnostic probes norcarane, 1,1-dimethylcyclopropane, and 1,1-diethylcyclopropane (2004), Biochemistry, 43, 15688-15701.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.236 T201A parameters similar to wild-type Pseudomonas mendocina
1.14.13.236 T201L parameters similar to wild-type Pseudomonas mendocina
1.14.13.236 T201S parameters similar to wild-type Pseudomonas mendocina

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.236 Pseudomonas mendocina Q00456 and Q00457 and Q00460 Q00456, Q00457 and Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH, respectively
-
1.14.13.236 Pseudomonas mendocina KR1 Q00456 and Q00457 and Q00460 Q00456, Q00457 and Q00460 are components TmoA, TmoB and TmoE of diiron hydroxylase T4MOH, respectively
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.236 1,1-diethylcyclopropane + NADH + H+ + O2
-
 Pseudomonas mendocina ? + NAD+ + H2O
-
 ?
1.14.13.236 1,1-diethylcyclopropane + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 ? + NAD+ + H2O
-
 ?
1.14.13.236 1,1-dimethylcyclopropane + NADH + H+ + O2
-
 Pseudomonas mendocina ? + NAD+ + H2O
-
 ?
1.14.13.236 1,1-dimethylcyclopropane + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 ? + NAD+ + H2O
-
 ?
1.14.13.236 additional information products are consistent with both radical rearrangement and cation ring expansion. Products show high-fidelity incorporation of an O-atom from O2 in the un-rearranged and radical-rearranged products, while the O-atom found in the cation ring-expansion products is predominantly obtained by reaction with H2O Pseudomonas mendocina ?
-
 ?
1.14.13.236 additional information products are consistent with both radical rearrangement and cation ring expansion. Products show high-fidelity incorporation of an O-atom from O2 in the un-rearranged and radical-rearranged products, while the O-atom found in the cation ring-expansion products is predominantly obtained by reaction with H2O Pseudomonas mendocina KR1 ?
-
 ?
1.14.13.236 norcarane + NADH + H+ + O2
-
 Pseudomonas mendocina endo-2-norcaranol + exo-2-norcaranol + endo-3-norcaranol + NAD+ + H2O 72% coupling, 47.5% endo-2-norcaranol, 39.2% exo-2- and endo-3-norcaranol, 8.8% exo-3-norcaranol ?
1.14.13.236 norcarane + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 endo-2-norcaranol + exo-2-norcaranol + endo-3-norcaranol + NAD+ + H2O 72% coupling, 47.5% endo-2-norcaranol, 39.2% exo-2- and endo-3-norcaranol, 8.8% exo-3-norcaranol ?
1.14.13.236 toluene + NADH + H+ + O2
-
 Pseudomonas mendocina 4-methylphenol + NAD+ + H2O 94% coupling ?
1.14.13.236 toluene + NADH + H+ + O2
-
 Pseudomonas mendocina KR1 4-methylphenol + NAD+ + H2O 94% coupling ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.236 T4MOH
-
 Pseudomonas mendocina

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.236 0.35
-
 norcarane mutant T201S, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 0.4
-
 norcarane mutant T201A, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 0.46
-
 norcarane mutant T201L, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 0.49
-
 norcarane wild-type, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 1.9
-
 Toluene mutant T201L, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 1.9
-
 Toluene mutant T201S, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 2.3
-
 Toluene wild-type, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 2.3
-
 Toluene mutant T201A, pH 7.5, 25°C Pseudomonas mendocina