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Literature summary extracted from

  • Pikus, J.D.; Mitchell, K.H.; Studts, J.M.; McClay, K.; Steffan, R.J.; Fox, B.G.
    Threonine 201 in the diiron enzyme toluene 4-monooxygenase is not required for catalysis (2000), Biochemistry, 39, 791-799.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.236 T201A mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution Pseudomonas mendocina
1.14.13.236 T201F mutation causes a substantial shift in the product distribution, and gives o- and p-cresol in a 1:1 ratio Pseudomonas mendocina
1.14.13.236 T201G mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution Pseudomonas mendocina
1.14.13.236 T201K decrease in activity Pseudomonas mendocina
1.14.13.236 T201S mutant retains catalytic activity and exhibits 80-90% coupling efficiency compared to 94% for wild-type, with p-cresol representing 90-95% of the total product distribution Pseudomonas mendocina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.236 0.005
-
Toluene wild-type, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 0.006
-
Toluene mutant T201A, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 0.006
-
Toluene mutant T201S, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 0.009
-
Toluene mutant T201G, pH 7.5, 25°C Pseudomonas mendocina

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.236 Iron 2.2 mol of Fe per mol of purified alphabetagamma protomer Pseudomonas mendocina

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.236 Pseudomonas mendocina Q00456 subunit TmoA
-
1.14.13.236 Pseudomonas mendocina KR1 Q00456 subunit TmoA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.236 toluene + NADH + H+ + O2
-
Pseudomonas mendocina 4-methylphenol + NAD+ + H2O 96% 4-methylphenol plus 2.8% 3-methylphenol plus 0.4% 2-methylphenol plus 0.8% benzyl alcohol ?
1.14.13.236 toluene + NADH + H+ + O2
-
Pseudomonas mendocina KR1 4-methylphenol + NAD+ + H2O 96% 4-methylphenol plus 2.8% 3-methylphenol plus 0.4% 2-methylphenol plus 0.8% benzyl alcohol ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.236 0.6
-
Toluene mutant T201G, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 1
-
Toluene mutant T201A, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 1.2
-
Toluene mutant T201S, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 1.3
-
Toluene wild-type, pH 7.5, 25°C Pseudomonas mendocina

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.13.236 70
-
Toluene mutant T201G, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 170
-
Toluene mutant T201A, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 200
-
Toluene mutant T201S, pH 7.5, 25°C Pseudomonas mendocina
1.14.13.236 300
-
Toluene wild-type, pH 7.5, 25°C Pseudomonas mendocina