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Literature summary extracted from

  • Tishkov, V.I.; Goncharenko, K.V.; Alekseeva, A.A.; Kleymenov, S.Y.; Savin, S.S.
    Role of a structurally equivalent phenylalanine residue in catalysis and thermal stability of formate dehydrogenases from different sources (2015), Biochemistry (Moscow), 80, 1690-1700.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.17.1.9 F290A the thermal stability of the mutant increases 1.3fold compared to the wild type enzyme Glycine max
1.17.1.9 F290D the thermal stability of the mutant increases 61fold compared to the wild type enzyme Glycine max
1.17.1.9 F290E the thermal stability of the mutant increases 55fold compared to the wild type enzyme Glycine max
1.17.1.9 F290N the thermal stability of the mutant increases 12.3fold compared to the wild type enzyme Glycine max
1.17.1.9 F290Q the thermal stability of the mutant increases 5.1fold compared to the wild type enzyme Glycine max
1.17.1.9 F290S the thermal stability of the mutant increases 4.8fold compared to the wild type enzyme Glycine max
1.17.1.9 F290T the thermal stability of the mutant increases 5fold compared to the wild type enzyme Glycine max
1.17.1.9 F290Y the thermal stability of the mutant increases 1.4fold compared to the wild type enzyme Glycine max
1.17.1.9 F311D the thermal stability of the mutant increases 2.42fold compared to the wild type enzyme Pseudomonas sp.
1.17.1.9 F311N the thermal stability of the mutant decreases 0.49fold compared to the wild type enzyme Pseudomonas sp.
1.17.1.9 F311S the thermal stability of the mutant decreases 0.52fold compared to the wild type enzyme Pseudomonas sp.
1.17.1.9 F311Y the thermal stability of the mutant increases 1.59fold compared to the wild type enzyme Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.0086
-
 NAD+ mutant enzyme F290A, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 0.0091
-
 NAD+ mutant enzyme F290S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 0.0109
-
 NAD+ mutant enzyme F290Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 0.0117
-
 NAD+ mutant enzyme F290Q, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 0.0128
-
 NAD+ mutant enzyme F290D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 0.0133
-
 NAD+ wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 0.0137
-
 NAD+ mutant enzyme F290E, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 0.014
-
 NAD+ mutant enzyme F290N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 0.0143
-
 NAD+ mutant enzyme F290T, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 0.041
-
 NAD+ wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.
1.17.1.9 0.055
-
 NAD+ mutant enzyme F311Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.
1.17.1.9 0.168
-
 NAD+ mutant enzyme F311N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.
1.17.1.9 0.17
-
 NAD+ mutant enzyme F311S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.
1.17.1.9 0.23
-
 NAD+ mutant enzyme F311D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.
1.17.1.9 0.9
-
 formate mutant enzyme F290Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 1.1
-
 formate mutant enzyme F290A, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 1.2
-
 formate mutant enzyme F290Q, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 1.3
-
 formate mutant enzyme F290T, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 1.5
-
 formate wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 2.9
-
 formate mutant enzyme F290E, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 3.2
-
 formate wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.
1.17.1.9 3.7
-
 formate mutant enzyme F311Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.
1.17.1.9 4.1
-
 formate mutant enzyme F290S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 4.5
-
 formate mutant enzyme F290N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 5
-
 formate mutant enzyme F290D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 19.2
-
 formate mutant enzyme F311N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.
1.17.1.9 22.7
-
 formate mutant enzyme F311S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.
1.17.1.9 29.8
-
 formate mutant enzyme F311D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.9 formate + NAD+ Glycine max
-
 CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+ Pseudomonas sp.
-
 CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Glycine max
-
-
-
1.17.1.9 Pseudomonas sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+
-
 Glycine max CO2 + NADH + H+
-
 ?
1.17.1.9 formate + NAD+
-
 Pseudomonas sp. CO2 + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.1.9 FDH
-
 Glycine max
1.17.1.9 FDH
-
 Pseudomonas sp.
1.17.1.9 NAD+-dependent formate dehydrogenase
-
 Glycine max
1.17.1.9 NAD+-dependent formate dehydrogenase
-
 Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.1.9 2.8
-
 NAD+ mutant enzyme F290N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 2.9
-
 NAD+ wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 3.5
-
 NAD+ mutant enzyme F290Q, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 3.5
-
 NAD+ mutant enzyme F290Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 3.8
-
 NAD+ mutant enzyme F290A, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 4
-
 NAD+ mutant enzyme F290T, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 4
-
 NAD+ mutant enzyme F311D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.
1.17.1.9 4.1
-
 NAD+ mutant enzyme F290S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 4.7
-
 NAD+ mutant enzyme F290E, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 5.1
-
 NAD+ mutant enzyme F290D, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Glycine max
1.17.1.9 5.4
-
 NAD+ mutant enzyme F311S, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.
1.17.1.9 5.5
-
 NAD+ mutant enzyme F311N, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.
1.17.1.9 7.3
-
 NAD+ mutant enzyme F311Y, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.
1.17.1.9 7.3
-
 NAD+ wild type enzyme, in 0.1 M sodium phosphate buffer, 0.01 M EDTA, pH 7.0, temperature not specified in the publication Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+
-
 Glycine max
1.17.1.9 NAD+
-
 Pseudomonas sp.