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Literature summary extracted from

  • Blaise, M.; Van Wyk, N.; Baneres-Roquet, F.; Guerardel, Y.; Kremer, L.
    Binding of NADP+ triggers an open-to-closed transition in a mycobacterial FabG beta-ketoacyl-ACP reductase (2017), Biochem. J., 474, 907-921.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.100 expressed in Escherichia coli BL21 Rosetta 2 (DE3) pLysS cells Mycolicibacterium smegmatis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.100 hanging drop vapor diffusion method, apoenzyme is crystallized by using 100 mM Na-HEPES (pH 7.5), 30% (w/v) PEG 400 and 200 mM magnesium chloride hexahydrate, while the complex with NADP+ is crystallized by using 200 mM sodium citrate tribasic dehydrate and 20% (w/v) PEG 3350 Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.100 100000
-
 x * 100000, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.100 beta-ketoacyl-[acyl-carrier protein] + NADPH + H+ Mycolicibacterium smegmatis
-
 beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 beta-ketoacyl-[acyl-carrier protein] + NADPH + H+ Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
 beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.100 Mycolicibacterium smegmatis A0R723
-
-
1.1.1.100 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 A0R723
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.100 nickel Sepharose column chromatography and ENrich SEC 650 gel filtration Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.100 acetoacetyl-CoA + NADPH + H+
-
 Mycolicibacterium smegmatis ?
-
 ?
1.1.1.100 acetoacetyl-CoA + NADPH + H+
-
 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ?
-
 ?
1.1.1.100 beta-ketoacyl-[acyl-carrier protein] + NADPH + H+
-
 Mycolicibacterium smegmatis beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 beta-ketoacyl-[acyl-carrier protein] + NADPH + H+
-
 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 beta-hydroxyacyl-[acyl-carrier protein] + NADP+
-
 ?
1.1.1.100 additional information no activity with NADH Mycolicibacterium smegmatis ?
-
 ?
1.1.1.100 additional information no activity with NADH Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.100 ? x * 100000, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
1.1.1.100 beta-ketoacyl-ACP reductase
-
 Mycolicibacterium smegmatis
1.1.1.100 FabG
-
 Mycolicibacterium smegmatis
1.1.1.100 ketoacyl-acyl carrier protein reductase
-
 Mycolicibacterium smegmatis
1.1.1.100 MSMEG_6753
-
 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.100 NADPH
-
 Mycolicibacterium smegmatis