Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hult, E.L.; Katouno, F.; Uchiyama, T.; Watanabe, T.; Sugiyama, J.
    Molecular directionality in crystalline beta-chitin: hydrolysis by chitinases A and B from Serratia marcescens 2170 (2005), Biochem. J., 388, 851-856.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.200 expression in Escherichia coli Serratia marcescens
3.2.1.201 expression in Escherichia coli Serratia marcescens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.200 ChiB comprises a catalytic (beta/alpha)8 barrel domain and a C-terminal putative chitin-binding domain. The cocrystallized chito-oligosaccharide is inserted into the catalytic cleft from the non-reducing end when digested by ChiB Serratia marcescens
3.2.1.201 ChiA comprises an N-terminal domain, a catalytic (beta/alpha)8 barrel domain and a small (alpha +beta) domain, which is inserted into the (beta/alpha)8 barrel domain. The cocrystallized chito-oligosaccharide is inserted into the catalytic cleft from the reducing end when digested by ChiB Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.200 Serratia marcescens P11797
-
-
3.2.1.200 Serratia marcescens 2170 P11797
-
-
3.2.1.201 Serratia marcescens O83008
-
-
3.2.1.201 Serratia marcescens 2170 O83008
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.200 beta-chitin + H2O
-
 Serratia marcescens ? ChiB is active from the nonreducing end to the reducing end ?
3.2.1.200 beta-chitin + H2O
-
 Serratia marcescens 2170 ? ChiB is active from the nonreducing end to the reducing end ?
3.2.1.201 beta-chitin + H2O
-
 Serratia marcescens ? ChiA is active from the reducing end to the nonreducing end ?
3.2.1.201 beta-chitin + H2O
-
 Serratia marcescens 2170 ? ChiA is active from the reducing end to the nonreducing end ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.200 ChiB
-
 Serratia marcescens
3.2.1.201 ChiA
-
 Serratia marcescens

General Information

EC Number General Information Comment Organism
3.2.1.200 physiological function digestion of beta-chitin by ChiB occurs from the nonreducing end to the reducing end. Chitinases ChiA and ChiB show synergistic interactions in simultaneous degradation of beta-chitin, but not of glycol chitin Serratia marcescens
3.2.1.201 physiological function digestion of beta-chitin by ChiA occurs from the reducing end to the nonreducing end. Chitinases ChiA and ChiB show synergistic interactions in simultaneous degradation of beta-chitin, but not of glycol chitin Serratia marcescens