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Literature summary extracted from

  • Yamamoto, H.; Kudoh, M.
    Novel chiral tool, (R)-2-octanol dehydrogenase, from Pichia finlandica: Purification, gene cloning, and application for optically active alpha-haloalcohols (2013), Appl. Microbiol. Biotechnol., 97, 8087-8096.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.73 dithiothreitol 123% activity at 1 mM Ogataea wickerhamii
1.1.1.73 N-ethylmaleimide 147% activity at 1 mM Ogataea wickerhamii
1.1.1.73 o-phenanthroline 123% activity at 1 mM Ogataea wickerhamii
1.1.1.73 p-chloromercuribenzoate 118% activity at 0.05 mM Ogataea wickerhamii
1.1.1.73 phenylmethylsulfonyl fluoride 111% activity at 1 mM Ogataea wickerhamii
1.1.1.73 ZnSO4 152% activity at 1 mM Ogataea wickerhamii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.73 expressed in Escherichia coli JM109 cells Ogataea wickerhamii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.73 EDTA 80.6% residual activity at 1 mM Ogataea wickerhamii
1.1.1.73 Fe2+ 93.2% residual activity at 1 mM Ogataea wickerhamii
1.1.1.73 Hg+ 61.9% residual activity at 1 mM Ogataea wickerhamii
1.1.1.73 additional information not inhibited by Co2+, Zn2+, K+, Ni2+, Ba2+, NH2OH, and quercetin Ogataea wickerhamii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.73 Ca2+ 174% activity at 1 mM Ogataea wickerhamii
1.1.1.73 Cu2+ 100% activity at 1 mM Ogataea wickerhamii
1.1.1.73 Mg2+ 162% activity at 1 mM Ogataea wickerhamii
1.1.1.73 Mn2+ 185% activity at 1 mM Ogataea wickerhamii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.73 30000
-
 2 * 30000, SDS-PAGE Ogataea wickerhamii
1.1.1.73 83000
-
 gel filtration Ogataea wickerhamii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.73 Ogataea wickerhamii A0JC04
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.73 ammonium sulfate precipitation, phenyl-Toyopearl column chromatography, HiTrap Blue column chromatography, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration Ogataea wickerhamii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.73 0.0224
-
 crude extract, at pH 8.5 and 30°C Ogataea wickerhamii
1.1.1.73 91.1
-
 after 4070fold purification, at pH 8.5 and 30°C Ogataea wickerhamii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.73 (1R)-1-phenylethan-1-ol + NAD+ 255% activity compared to (R)-octan-2-ol Ogataea wickerhamii phenylacetaldehyde + NADH + H+
-
 ?
1.1.1.73 (1S)-1-phenylethan-1-ol + NAD+ 1.8% activity compared to (R)-octan-2-ol Ogataea wickerhamii phenylacetaldehyde + NADH + H+
-
 ?
1.1.1.73 (2R)-butan-2-ol + NAD+ 49.3% activity compared to (R)-octan-2-ol Ogataea wickerhamii butan-2-one + NADH + H+
-
 ?
1.1.1.73 (2R)-hexan-2-ol + NAD+ 54.1% activity compared to (R)-octan-2-ol Ogataea wickerhamii hexan-2-one + NADH + H+
-
 ?
1.1.1.73 (2S)-butan-2-ol + NAD+ 7.3% activity compared to (R)-octan-2-ol Ogataea wickerhamii butan-2-one + NADH + H+
-
 ?
1.1.1.73 (R)-octan-2-ol + NAD+ 100% activity Ogataea wickerhamii octan-2-one + NADH + H+
-
 ?
1.1.1.73 (RS)-octan-2-ol + NAD+ 77.8% activity compared to (R)-octan-2-ol Ogataea wickerhamii octan-2-one + NADH + H+
-
 ?
1.1.1.73 (S)-octan-2-ol + NAD+ 4.7% activity compared to (R)-octan-2-ol Ogataea wickerhamii octan-2-one + NADH + H+
-
 ?
1.1.1.73 1-phenoxypropan-2-ol + NAD+ 15.5% activity compared to (R)-octan-2-ol Ogataea wickerhamii 1-phenoxypropan-2-one + NADH + H+ +
-
 ?
1.1.1.73 1-phenoxypropan-2-one + NADH + H+ 54.2% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 1-phenoxypropan-2-ol + NAD+
-
 ?
1.1.1.73 1-phenylethan-1-one + NADH + H+ 45.3% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 1-phenylethan-1-ol + NAD+
-
 ?
1.1.1.73 2-bromo-1-cyclopropylethan-1-one + NADH + H+ 54.3% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 2-bromo-1-cyclopropylethan-1-ol + NAD+
-
 ?
1.1.1.73 2-chloro-1-(3-chlorophenyl)ethan-1-one + NADH + H+ 64.7% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 2-chloro-1-(3-chlorophenyl)ethan-1-ol + NAD+
-
 ?
1.1.1.73 2-chloro-1-(4-fluorophenyl)ethan-1-one + NADH + H+ 89.2% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 2-chloro-1-(4-fluorophenyl)ethan-1-ol + NAD+
-
 ?
1.1.1.73 2-chloro-1-(pyridin-3-yl)ethan-1-one + NADH + H+ 9.4% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 2-chloro-1-(pyridin-3-yl)ethan-1-ol + NAD+
-
 ?
1.1.1.73 2-chloro-1-phenylethan-1-one + NADH + H+ 52.6% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 2-chloro-1-phenylethan-1-ol + NAD+
-
 ?
1.1.1.73 3-acetyloxolan-2-one + NADH + H+ 12.7% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 3-acetyloxolan-2-ol + NAD+
-
 ?
1.1.1.73 5-chloropentan-2-one + NADH + H+ 108% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii 5-chloropentan-2-ol + NAD+
-
 ?
1.1.1.73 butan-2-one + NADH + H+ 8.0% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii butan-2-ol + NAD+
-
 ?
1.1.1.73 ethanol + NAD+ 0.7% activity compared to (R)-octan-2-ol Ogataea wickerhamii acetaldehyde + NADH + H+
-
 ?
1.1.1.73 ethyl (3R)-3-hydroxybutanoate + NAD+ 41.1% activity compared to (R)-octan-2-ol Ogataea wickerhamii ethyl 3-oxobutanoate + NADH + H+
-
 ?
1.1.1.73 ethyl (3S)-3-hydroxybutanoate + NAD+ 1.0% activity compared to (R)-octan-2-ol Ogataea wickerhamii ethyl 3-oxobutanoate + NADH + H+
-
 ?
1.1.1.73 ethyl (3S)-4-chloro-3-hydroxybutanoate + NAD+ 2.2% activity compared to (R)-octan-2-ol Ogataea wickerhamii ethyl 4-chloro-3-oxobutanoate + NADH + H+
-
 ?
1.1.1.73 ethyl 2-chloro-3-oxobutanoate + NADH + H+ 571% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii ethyl 2-chloro-3-oxobutanoate + NAD+
-
 ?
1.1.1.73 ethyl 3-oxobutanoate + NADH + H+ 105.8% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii ethyl 3-hydroxybutanoate + NAD+
-
 ?
1.1.1.73 ethyl 4-chloroacetoacetate + NADH + H+ 100% activity Ogataea wickerhamii ?
-
 ?
1.1.1.73 octan-1-ol + NAD+ 1.7% activity compared to (R)-octan-2-ol Ogataea wickerhamii octanal + NADH + H+
-
 ?
1.1.1.73 octan-2-one + NADH + H+ 31.2% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii octan-2-ol + NAD+
-
 ?
1.1.1.73 propan-2-ol + NAD+ 6.8% activity compared to (R)-octan-2-ol Ogataea wickerhamii propan-2-one + NADH + H+
-
 ?
1.1.1.73 propan-2-one + NADH + H+ 1.8% activity compared to ethyl 4-chloroacetoacetate Ogataea wickerhamii propan-2-ol + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.73 homotrimer 2 * 30000, SDS-PAGE Ogataea wickerhamii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.73 (R)-2-octanol dehydrogenase
-
 Ogataea wickerhamii
1.1.1.73 ODH
-
 Ogataea wickerhamii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.73 50
-
 oxidation reaction Ogataea wickerhamii
1.1.1.73 50 55 reduction reaction Ogataea wickerhamii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.73 6
-
 reduction reaction Ogataea wickerhamii
1.1.1.73 10.5
-
 oxidation reaction Ogataea wickerhamii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.73 NAD+
-
 Ogataea wickerhamii