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Literature summary extracted from

  • Rao, G.; Lee, J.K.; Zhao, H.
    Directed evolution of phloroglucinol synthase PhlD with increased stability for phloroglucinol production (2013), Appl. Microbiol. Biotechnol., 97, 5861-5867.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.253 expressed in Escherichia coli BL21(DE3) cells Pseudomonas fluorescens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.253 A181S the mutant shows 110% of wild type specific activity Pseudomonas fluorescens
2.3.1.253 A82T the mutant shows wild type specific activity Pseudomonas fluorescens
2.3.1.253 L136M/S243T the mutant shows 90% of wild type specific activity Pseudomonas fluorescens
2.3.1.253 L54V the mutant shows 110% of wild type specific activity Pseudomonas fluorescens
2.3.1.253 L54V/A82T the mutant shows wild type specific activity Pseudomonas fluorescens
2.3.1.253 M21T the mutant shows 90% of wild type specific activity Pseudomonas fluorescens
2.3.1.253 M21T/L54V/A82T the mutant shows 90% of wild type specific activity Pseudomonas fluorescens
2.3.1.253 M21T/L54V/A82T/A181S the mutant shows wild type specific activity Pseudomonas fluorescens
2.3.1.253 M21T/L54V/A82T/S96R/A181S the mutant shows 110% of wild type specific activity Pseudomonas fluorescens
2.3.1.253 M21T/N27D/A82T/A181S the mutant shows 90% of wild type specific activity Pseudomonas fluorescens
2.3.1.253 M21T/N27D/L54V/A82T/A181S/S243T the mutant shows wild type specific activity Pseudomonas fluorescens
2.3.1.253 M21T/N27D/L54V/A82T/L136M/A181S/S243T the mutant shows 110% of wild type specific activity Pseudomonas fluorescens
2.3.1.253 N27D the mutant shows 90% of wild type specific activity Pseudomonas fluorescens
2.3.1.253 S96R the mutant shows 80% of wild type specific activity Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.253 3 malonyl-CoA Pseudomonas fluorescens
-
phloroglucinol + 3 CO2 + 3 CoA phloroglucinol is 1,3,5-trihydroxylbenzene ?
2.3.1.253 3 malonyl-CoA Pseudomonas fluorescens Pf-5
-
phloroglucinol + 3 CO2 + 3 CoA phloroglucinol is 1,3,5-trihydroxylbenzene ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.253 Pseudomonas fluorescens
-
-
-
2.3.1.253 Pseudomonas fluorescens Pf-5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.253 Ni-NTA column chromatography Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.253 3 malonyl-CoA
-
Pseudomonas fluorescens phloroglucinol + 3 CO2 + 3 CoA phloroglucinol is 1,3,5-trihydroxylbenzene ?
2.3.1.253 3 malonyl-CoA
-
Pseudomonas fluorescens Pf-5 phloroglucinol + 3 CO2 + 3 CoA phloroglucinol is 1,3,5-trihydroxylbenzene ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.253 PhlD
-
Pseudomonas fluorescens
2.3.1.253 phloroglucinol synthase
-
Pseudomonas fluorescens