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Literature summary extracted from

  • Hong, S.H.; Ngo, H.P.; Nam, H.K.; Kim, K.R.; Kang, L.W.; Oh, D.K.
    Alternative biotransformation of retinal to retinoic acid or retinol by an aldehyde dehydrogenase from Bacillus cereus (2016), Appl. Environ. Microbiol., 82, 3940-3946.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.300 expressed in Escherichia coli ER2566 cells Bacillus cereus
1.2.1.5 expressed in Escherichia coli ER2566 cells Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.300 C300A the mutant shows no oxidation activity Bacillus cereus
1.1.1.300 C300S the mutant shows reduced catalytic efficiency for the oxidation and reduction of all-trans-retinal compared to the wild-type enzyme Bacillus cereus
1.1.1.300 E194S the mutant shows 15fold higher catalytic efficiency for the reduction of all-trans-retinal than the wild-type enzyme Bacillus cereus
1.1.1.300 E266A the mutant shows no oxidation activity Bacillus cereus
1.1.1.300 E457V the mutant shows 7.5fold higher catalytic efficiency for the reduction of all-trans-retinal than the wild-type enzyme Bacillus cereus
1.2.1.5 C300A the variant shows no oxidation activity Bacillus cereus
1.2.1.5 E194S the variant shows 15fold higher catalytic efficiency (kcat/Km) for the reduction of all-trans-retinal than the wild type enzyme Bacillus cereus
1.2.1.5 E266A the variant shows no oxidation activity Bacillus cereus
1.2.1.5 E457V the variant shows 7.5fold higher catalytic efficiency (kcat/Km) for the reduction of all-trans-retinal than the wild type enzyme Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.300 0.004
-
 NAD+ at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.0042
-
 NADP+ at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.007
-
 retinal with NAD+ as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.04
-
 NADPH at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.13
-
 retinal with NADPH as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.0037
-
 NAD+ mutant enzyme C300S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.004
-
 NAD+ wild type enzyme, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.00625
-
 NAD+ mutant enzyme E457V, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.007
-
 all-trans-retinal with NAD+ as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.01
-
 NAD+ mutant enzyme E194S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.095
-
 acetaldehyde at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.2
-
 benzaldehyde at pH 7.0 and 37°C Bacillus cereus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.5 54000
-
 4 * 54000, SDS-PAGE Bacillus cereus
1.2.1.5 216000
-
 gel filtration Bacillus cereus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.300 Bacillus cereus
-
-
-
1.2.1.5 Bacillus cereus A0A150BLG9
-
-
1.2.1.5 Bacillus cereus ATCC 10876 A0A150BLG9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.300 Sephacryl S-300 gel filtration Bacillus cereus
1.2.1.5 Sephacryl S-300 gel filtration Bacillus cereus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.300 0.012
-
 mutant enzyme C300S, with retinal and NADPH as substrates, at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.0386
-
 mutant enzyme C300S, with retinal and NAD+ as substrates, at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.068
-
 mutant enzyme C300A, with retinal and NADPH as substrates, at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.08
-
 mutant enzyme E266A, with retinal and NADPH as substrates, at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.136
-
 wild type enzyme, with retinal and NADPH as substrates, at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.26
-
 mutant enzyme E457V, with retinal and NADPH as substrates, at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.408
-
 mutant enzyme E194S, with retinal and NADPH as substrates, at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.461
-
 wild type enzyme, with retinal and NAD+ as substrates, at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.493
-
 mutant enzyme E194S, with retinal and NAD+ as substrates, at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 2.536
-
 mutant enzyme E457V, with retinal and NAD+ as substrates, at pH 7.0 and 37°C Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.300 additional information no activity with 9-cis-retinal and 13-cis-retinal Bacillus cereus ?
-
 ?
1.1.1.300 retinal + NAD+
-
 Bacillus cereus retinoic acid + NADH + H+
-
 ?
1.1.1.300 retinal + NADPH + H+
-
 Bacillus cereus retinol + NADP+
-
 ?
1.1.1.300 retinol + NADP+
-
 Bacillus cereus retinal + NADPH + H+
-
 ?
1.2.1.5 acetaldehyde + NAD+ + H2O
-
 Bacillus cereus acetate + NADH + H+
-
 ?
1.2.1.5 acetaldehyde + NAD+ + H2O
-
 Bacillus cereus ATCC 10876 acetate + NADH + H+
-
 ?
1.2.1.5 all-trans-retinal + NAD+ + H2O
-
 Bacillus cereus all-trans-retinoic acid + NADH + H+
-
 ?
1.2.1.5 all-trans-retinal + NAD+ + H2O
-
 Bacillus cereus ATCC 10876 all-trans-retinoic acid + NADH + H+
-
 ?
1.2.1.5 all-trans-retinal + NADP+ + H2O
-
 Bacillus cereus all-trans-retinol + NADPH + H+
-
 ?
1.2.1.5 all-trans-retinal + NADP+ + H2O
-
 Bacillus cereus ATCC 10876 all-trans-retinol + NADPH + H+
-
 ?
1.2.1.5 benzaldehyde + NAD+ + H2O
-
 Bacillus cereus benzoate + NADH + H+
-
 ?
1.2.1.5 benzaldehyde + NAD+ + H2O
-
 Bacillus cereus ATCC 10876 benzoate + NADH + H+
-
 ?
1.2.1.5 additional information no activity with 9-cis-retinal and 13-cis-retinal Bacillus cereus ?
-
 ?
1.2.1.5 additional information no activity with 9-cis-retinal and 13-cis-retinal Bacillus cereus ATCC 10876 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.5 homotetramer 4 * 54000, SDS-PAGE Bacillus cereus

Synonyms

EC Number Synonyms Comment Organism
1.1.1.300 aldehyde dehydrogenase
-
 Bacillus cereus
1.1.1.300 ALDH
-
 Bacillus cereus
1.2.1.5 ALDH
-
 Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.300 0.00055
-
 NADPH at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.0017
-
 NAD+ at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.0018
-
 NADP+ at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.002
-
 retinal with NADPH as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.0047
-
 retinal with NAD+ as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.0007
-
 NAD+ mutant enzyme C300S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.0017
-
 NAD+ wild type enzyme, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.005
-
 NAD+ mutant enzyme E194S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.005
-
 all-trans-retinal with NAD+ as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.026
-
 NAD+ mutant enzyme E457V, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.183
-
 acetaldehyde at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.217
-
 benzaldehyde at pH 7.0 and 37°C Bacillus cereus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.300 NAD+
-
 Bacillus cereus
1.1.1.300 NADH
-
 Bacillus cereus
1.1.1.300 NADP+
-
 Bacillus cereus
1.1.1.300 NADPH
-
 Bacillus cereus
1.2.1.5 NAD+
-
 Bacillus cereus
1.2.1.5 NADP+
-
 Bacillus cereus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.300 0.014
-
 NADPH at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.015
-
 retinal with NADPH as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.417
-
 NAD+ at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.433
-
 NADP+ at pH 7.0 and 37°C Bacillus cereus
1.1.1.300 0.667
-
 retinal with NAD+ as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.16
-
 NAD+ mutant enzyme C300S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.42
-
 NAD+ wild type enzyme, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.5
-
 NAD+ mutant enzyme E194S, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 0.67
-
 all-trans-retinal with NAD+ as cosubstrate, at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 1.08
-
 benzaldehyde at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 1.85
-
 acetaldehyde at pH 7.0 and 37°C Bacillus cereus
1.2.1.5 4.1
-
 NAD+ mutant enzyme E457V, with all-trans-retinal as cosubstrate, at pH 7.0 and 37°C Bacillus cereus