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Literature summary extracted from

  • Eprintsev, A.; Gataullina, M.; Lyashchenko, M.
    Physicochemical and catalytic properties of NAD+-dependent malate dehydrogenase isoforms from maize mesophyll (2016), Appl. Biochem. Microbiol., 52, 366-370.
No PubMed abstract available

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 31600
-
-
 Zea mays
1.1.1.37 31600
-
 2 * 31600, SDS-PAGE Zea mays
1.1.1.37 63300
-
 enzyme form 2, gel fitration Zea mays
1.1.1.37 126600
-
 enzyme form 1, gel fitration Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 (S)-malate + NAD+ Zea mays
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH + H+ Zea mays
-
 (S)-malate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.37 native tetrameric and dimeric MDH enzyme forms, 46 and 71fold, respectively, from mesophyll of maize leaves to homogeneity by ammonium sulfate fractionation, gel filtration, and anion excange chromatography Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 leaf
-
 Zea mays
-
1.1.1.37 mesophyll
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.37 640
-
 purified native enzyme form 1, malate oxidation, pH 8.0, 25°C Zea mays
1.1.1.37 990
-
 purified native enzyme form 2, malate oxidation, pH 8.0, 25°C Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Zea mays oxaloacetate + NADH + H+
-
 r
1.1.1.37 additional information the tetrameric MDH enzyme form has a higher affinity for NADH and oxaloacetate, and the dimeric has a higher affinity for NAD+ and malate Zea mays ?
-
 ?
1.1.1.37 oxaloacetate + NADH + H+
-
 Zea mays (S)-malate + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.37 dimer 2 * 31600, SDS-PAGE Zea mays
1.1.1.37 tetramer 4 * 31600, SDS-PAGE Zea mays

Synonyms

EC Number Synonyms Comment Organism
1.1.1.37 MDH
-
 Zea mays
1.1.1.37 NAD+-dependent malate dehydrogenase
-
 Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.37 25
-
 both reaction directions, assay at Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.37 7.5 8.5 oxaloacetate reduction Zea mays
1.1.1.37 8.5 9.5 malate oxidation Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.37 NAD+
-
 Zea mays
1.1.1.37 NADH
-
 Zea mays

General Information

EC Number General Information Comment Organism
1.1.1.37 metabolism the enzyme is involved in the Krebs cycle (catabolism), glyoxylate and Hatch-Slack cycles, and malate metabolism, as well as other anabolic processes Zea mays