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Literature summary extracted from

  • Pongtharangkul, T.; Chuekitkumchorn, P.; Suwanampa, N.; Payongsri, P.; Honda, K.; Panbangred, W.
    Kinetic properties and stability of glucose dehydrogenase from Bacillus amyloliquefaciens SB5 and its potential for cofactor regeneration (2015), AMB Express, 5, 68.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.47 expressed in Escherichia coli BL21(DE3) cells Bacillus amyloliquefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.47 acetone 1% residual activity at 50% (v/v) Bacillus amyloliquefaciens
1.1.1.47 ethanol 19% residual activity at 50% (v/v) Bacillus amyloliquefaciens
1.1.1.47 n-butanol 1% residual activity at 50% (v/v) Bacillus amyloliquefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 0.05
-
 NADP+ at pH 8.0 and 37°C Bacillus amyloliquefaciens
1.1.1.47 0.25
-
 NAD+ at pH 8.0 and 37°C Bacillus amyloliquefaciens
1.1.1.47 5.5
-
 D-glucose with NADP+ as cosubstrate,at pH 8.0 and 37°C Bacillus amyloliquefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.47 28000
-
-
 Bacillus amyloliquefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.47 D-glucose + NAD+ Bacillus amyloliquefaciens
-
 D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NAD+ Bacillus amyloliquefaciens SB5
-
 D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NADP+ Bacillus amyloliquefaciens
-
 D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 D-glucose + NADP+ Bacillus amyloliquefaciens SB5
-
 D-glucono-1,5-lactone + NADPH + H+
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.1.1.47 1-hexanol the enzyme exhibits an extreme tolerance towards 1-hexanol with 6%loss of activity at 10% (v/v) Bacillus amyloliquefaciens
1.1.1.47 2-hexanol the enzyme exhibits an extreme tolerance towards 2-hexanol with 7%loss of activity at 50% (v/v) Bacillus amyloliquefaciens
1.1.1.47 DMSO the enzyme exhibits an extreme tolerance towards DMSO with 1% loss of activity at 50% (v/v) Bacillus amyloliquefaciens
1.1.1.47 n-hexane the enzyme exhibits an extreme tolerance towards n-hexane with no loss of activity at 50% (v/v) Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Bacillus amyloliquefaciens
-
-
-
1.1.1.47 Bacillus amyloliquefaciens SB5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.47 PrepEase histidine-tagged protein purification kits Bacillus amyloliquefaciens

Storage Stability

EC Number Storage Stability Organism
1.1.1.47 -80°C, in CPB buffer (pH 6.0), at least 4 months, no loss of activity Bacillus amyloliquefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 D-glucose + NAD+
-
 Bacillus amyloliquefaciens D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NAD+
-
 Bacillus amyloliquefaciens SB5 D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NADP+
-
 Bacillus amyloliquefaciens D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 D-glucose + NADP+
-
 Bacillus amyloliquefaciens SB5 D-glucono-1,5-lactone + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.47 ? x * 28000, His6-tagged enzyme, SDS-PAGE Bacillus amyloliquefaciens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.47 glucose dehydrogenase
-
 Bacillus amyloliquefaciens
1.1.1.47 GluDH
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.47 50
-
-
 Bacillus amyloliquefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.47 42
-
 the enzyme is stable up to 42°C Bacillus amyloliquefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.47 70
-
 D-glucose at pH 8.0 and 37°C Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.47 10
-
-
 Bacillus amyloliquefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.47 5 8 when incubated for 6 h at 37°C, the enzyme is highly stable in acidic conditions (pH 5.0-6.0) but is completely inactivated at pH values above 8.0 Bacillus amyloliquefaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.47 NAD+
-
 Bacillus amyloliquefaciens
1.1.1.47 NADP+
-
 Bacillus amyloliquefaciens