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Literature summary extracted from

  • Alekseeva, A.A.; Kargov, I.S.; Kleimenov, S.Y.; Savin, S.S.; Tishkov, V.I.
    Additivity of the stabilization effect of single amino acid substitutions in triple mutants of recombinant formate dehydrogenase from the soybean Glycine max (2015), Acta Naturae, 7, 55-64.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.9 expressed in Escherichia coli BL21(DE3) Codon Plus/pLysS cells Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
1.17.1.9 A267M the mutant shows increased catalytic efficiency compared to the wild type enzyme Glycine max
1.17.1.9 A267M/I272V the mutant shows reduced catalytic efficiency compared to the wild type enzyme Glycine max
1.17.1.9 A267M/I272V/F290D the mutant shows reduced catalytic efficiency compared to the wild type enzyme Glycine max
1.17.1.9 A267M/I272V/F290N the mutant shows reduced catalytic efficiency compared to the wild type enzyme Glycine max
1.17.1.9 A267M/I272V/F290S the mutant shows reduced catalytic efficiency compared to the wild type enzyme Glycine max
1.17.1.9 F290D the mutant shows reduced catalytic efficiency compared to the wild type enzyme Glycine max
1.17.1.9 F290N the mutant shows reduced catalytic efficiency compared to the wild type enzyme Glycine max
1.17.1.9 F290S the mutant shows reduced catalytic efficiency compared to the wild type enzyme Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 1.5
-
 formate wild type enzyme, at pH 8.0 and 37°C Glycine max
1.17.1.9 2.1
-
 formate mutant enzyme A267M, at pH 8.0 and 37°C Glycine max
1.17.1.9 2.2
-
 formate mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C Glycine max
1.17.1.9 2.3
-
 formate mutant enzyme A267M/I272V/F290S, at pH 8.0 and 37°C Glycine max
1.17.1.9 2.4
-
 formate mutant enzyme A267M/I272V, at pH 8.0 and 37°C Glycine max
1.17.1.9 2.8
-
 formate mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C Glycine max
1.17.1.9 4.1
-
 formate mutant enzyme F290S, at pH 8.0 and 37°C Glycine max
1.17.1.9 4.5
-
 formate mutant enzyme F290N, at pH 8.0 and 37°C Glycine max
1.17.1.9 5
-
 formate mutant enzyme F290D, at pH 8.0 and 37°C Glycine max
1.17.1.9 9.1
-
 NAD+ mutant enzyme F290S, at pH 8.0 and 37°C Glycine max
1.17.1.9 9.9
-
 NAD+ mutant enzyme A267M, at pH 8.0 and 37°C Glycine max
1.17.1.9 12.8
-
 NAD+ mutant enzyme F290D, at pH 8.0 and 37°C Glycine max
1.17.1.9 13.3
-
 NAD+ wild type enzyme, at pH 8.0 and 37°C Glycine max
1.17.1.9 13.3
-
 NAD+ mutant enzyme A267M/I272V, at pH 8.0 and 37°C Glycine max
1.17.1.9 14
-
 NAD+ mutant enzyme F290N, at pH 8.0 and 37°C Glycine max
1.17.1.9 14.1
-
 NAD+ mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C Glycine max
1.17.1.9 16.1
-
 NAD+ mutant enzyme A267M/I272V/F290S, at pH 8.0 and 37°C Glycine max
1.17.1.9 20.3
-
 NAD+ mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C Glycine max

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 Glycine max
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.9 ammonium sulfate precipitation, phenyl Sepharose column chromatography, and Sephadex G-25 gel filtration Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+
-
 Glycine max CO2 + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.1.9 NAD(P)+.dependent formate dehydrogenase
-
 Glycine max

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.9 57
-
 melting temperature Glycine max

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.1.9 2.2
-
 formate mutant enzyme A267M/I272V, at pH 8.0 and 37°C Glycine max
1.17.1.9 2.8
-
 formate mutant enzyme F290N, at pH 8.0 and 37°C Glycine max
1.17.1.9 2.9
-
 formate wild type enzyme, at pH 8.0 and 37°C Glycine max
1.17.1.9 2.9
-
 formate mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C Glycine max
1.17.1.9 3.2
-
 formate mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C Glycine max
1.17.1.9 3.7
-
 formate mutant enzyme A267M/I272V/F290S, at pH 8.0 and 37°C Glycine max
1.17.1.9 4.1
-
 formate mutant enzyme F290S, at pH 8.0 and 37°C Glycine max
1.17.1.9 5
-
 formate mutant enzyme A267M, at pH 8.0 and 37°C Glycine max
1.17.1.9 5.1
-
 formate mutant enzyme F290D, at pH 8.0 and 37°C Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+
-
 Glycine max

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.17.1.9 0.62
-
 formate mutant enzyme F290D, at pH 8.0 and 37°C Glycine max
1.17.1.9 0.92
-
 formate mutant enzyme A267M/I272V, at pH 8.0 and 37°C Glycine max
1.17.1.9 1
-
 formate mutant enzyme F290S, at pH 8.0 and 37°C Glycine max
1.17.1.9 1.02
-
 formate mutant enzyme F290N, at pH 8.0 and 37°C Glycine max
1.17.1.9 1.36
-
 formate mutant enzyme A267M/I272V/F290S, at pH 8.0 and 37°C Glycine max
1.17.1.9 1.45
-
 formate mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C Glycine max
1.17.1.9 1.61
-
 formate mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C Glycine max
1.17.1.9 1.93
-
 formate wild type enzyme, at pH 8.0 and 37°C Glycine max
1.17.1.9 2.38
-
 formate mutant enzyme A267M, at pH 8.0 and 37°C Glycine max
1.17.1.9 140
-
 NAD+ mutant enzyme A267M/I272V/F290D, at pH 8.0 and 37°C Glycine max
1.17.1.9 170
-
 NAD+ mutant enzyme A267M/I272V, at pH 8.0 and 37°C Glycine max
1.17.1.9 200
-
 NAD+ mutant enzyme F290D, at pH 8.0 and 37°C Glycine max
1.17.1.9 220
-
 NAD+ wild type enzyme, at pH 8.0 and 37°C Glycine max
1.17.1.9 230
-
 NAD+ mutant enzyme A267M/I272V/F290N, at pH 8.0 and 37°C Glycine max
1.17.1.9 230
-
 NAD+ mutant enzyme A267M/I272V/F290S, at pH 8.0 and 37°C Glycine max
1.17.1.9 400
-
 NAD+ mutant enzyme F290N, at pH 8.0 and 37°C Glycine max
1.17.1.9 450
-
 NAD+ mutant enzyme F290S, at pH 8.0 and 37°C Glycine max
1.17.1.9 510
-
 NAD+ mutant enzyme A267M, at pH 8.0 and 37°C Glycine max