EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.8 | KCl | maximum activity at 0.5 M | Staphylococcus aureus | |
1.2.1.8 | NaCl | maximum activity at 0.5 M | Staphylococcus aureus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.8 | expressed in Escherichia coli BL21(DE3)/pMAGIC cells | Staphylococcus aureus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.2.1.8 | sitting drop vapor diffusion method, using 200 mM MgCl2,100 mM Tris pH 8.5, 20% (w/v) PEG 8000 | Staphylococcus aureus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.1.8 | G234S | the mutant shows increased activity and reduced substrate inhibition compared to the wild type enzyme | Staphylococcus aureus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.8 | Betaine aldehyde | strongly inhibited by betaine aldehyde at concentrations of more than 0.15 mM | Staphylococcus aureus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.8 | 0.17 | - |
Betaine aldehyde | at pH 8.0 and 30°C | Staphylococcus aureus | |
1.2.1.8 | 0.43 | - |
NAD+ | at pH 8.0 and 30°C | Staphylococcus aureus | |
1.2.1.8 | 2.26 | - |
NADP+ | at pH 8.0 and 30°C | Staphylococcus aureus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.8 | 60000 | - |
4 * 60000, SDS-PAGE | Staphylococcus aureus |
1.2.1.8 | 215000 | - |
gel filtration | Staphylococcus aureus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.8 | betaine aldehyde + NAD+ + H2O | Staphylococcus aureus | - |
betaine + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.8 | Staphylococcus aureus | Q9L4P8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.8 | Ni-NTA column chromatography and Superdex 200 gel filtration | Staphylococcus aureus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.8 | betaine aldehyde + NAD+ + H2O | - |
Staphylococcus aureus | betaine + NADH + H+ | - |
? | |
1.2.1.8 | betaine aldehyde + NADP+ + H2O | the enzyme is also active with NADP+, but the NAD-supported activity is at least 10times higher | Staphylococcus aureus | betaine + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.8 | homotetramer | 4 * 60000, SDS-PAGE | Staphylococcus aureus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.8 | BADH | - |
Staphylococcus aureus |
1.2.1.8 | SACOL2628 | - |
Staphylococcus aureus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.8 | 0.56 | - |
NADP+ | at pH 8.0 and 30°C | Staphylococcus aureus | |
1.2.1.8 | 6.18 | - |
NAD+ | at pH 8.0 and 30°C | Staphylococcus aureus | |
1.2.1.8 | 11 | - |
Betaine aldehyde | at pH 8.0 and 30°C | Staphylococcus aureus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.2.1.8 | 8 | - |
- |
Staphylococcus aureus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.8 | NAD+ | the enzyme is also active with NADP+, but the NAD-supported activity is at least 10times higher | Staphylococcus aureus | |
1.2.1.8 | NADP+ | the enzyme is also active with NADP+, but the NAD-supported activity is at least 10times higher | Staphylococcus aureus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.8 | 0.34 | - |
Betaine aldehyde | at pH 8.0 and 30°C | Staphylococcus aureus |