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Literature summary extracted from

  • Halavaty, A.S.; Rich, R.L.; Chen, C.; Joo, J.C.; Minasov, G.; Dubrovska, I.; Winsor, J.R.; Myszka, D.G.; Duban, M.; Shuvalova, L.; Yakunin, A.F.; Anderson, W.F.
    Structural and functional analysis of betaine aldehyde dehydrogenase from Staphylococcus aureus (2015), Acta Crystallogr. Sect. D, 71, 1159-1175.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.8 KCl maximum activity at 0.5 M Staphylococcus aureus
1.2.1.8 NaCl maximum activity at 0.5 M Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.8 expressed in Escherichia coli BL21(DE3)/pMAGIC cells Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.8 sitting drop vapor diffusion method, using 200 mM MgCl2,100 mM Tris pH 8.5, 20% (w/v) PEG 8000 Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.8 G234S the mutant shows increased activity and reduced substrate inhibition compared to the wild type enzyme Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.8 Betaine aldehyde strongly inhibited by betaine aldehyde at concentrations of more than 0.15 mM Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.8 0.17
-
Betaine aldehyde at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 0.43
-
NAD+ at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 2.26
-
NADP+ at pH 8.0 and 30°C Staphylococcus aureus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.8 60000
-
4 * 60000, SDS-PAGE Staphylococcus aureus
1.2.1.8 215000
-
gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O Staphylococcus aureus
-
betaine + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Staphylococcus aureus Q9L4P8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.8 Ni-NTA column chromatography and Superdex 200 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O
-
Staphylococcus aureus betaine + NADH + H+
-
?
1.2.1.8 betaine aldehyde + NADP+ + H2O the enzyme is also active with NADP+, but the NAD-supported activity is at least 10times higher Staphylococcus aureus betaine + NADPH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.8 homotetramer 4 * 60000, SDS-PAGE Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
1.2.1.8 BADH
-
Staphylococcus aureus
1.2.1.8 SACOL2628
-
Staphylococcus aureus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.8 0.56
-
NADP+ at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 6.18
-
NAD+ at pH 8.0 and 30°C Staphylococcus aureus
1.2.1.8 11
-
Betaine aldehyde at pH 8.0 and 30°C Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.8 8
-
-
Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD+ the enzyme is also active with NADP+, but the NAD-supported activity is at least 10times higher Staphylococcus aureus
1.2.1.8 NADP+ the enzyme is also active with NADP+, but the NAD-supported activity is at least 10times higher Staphylococcus aureus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.1.8 0.34
-
Betaine aldehyde at pH 8.0 and 30°C Staphylococcus aureus