Literature summary extracted from
Papanikolau, Y.; Petratos, K.
Application of the effects of ionic strength reducing agents in the purification and crystallization of chitinase A (2002), Acta Crystallogr. Sect. D, 58, 1593-1596.
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.2.1.201 |
ChiA crystallizes both by salting-in and salting-out in the presence of a variety of precipitating agents in a wide range of concentrations and pH values |
Serratia marcescens |
General Stability
EC Number |
General Stability |
Organism |
---|
3.2.1.201 |
protein precipitates at concentrations higher than 10 mg/ml in a variety of pH and ionic strength values |
Serratia marcescens |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.201 |
Serratia marcescens |
O83008 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.201 |
ChiA binds nonspecifically polysaccharides, which are found in the growth medium. These sugar impurities have to be removed during the purification process |
Serratia marcescens |