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Literature summary extracted from

  • Papanikolau, Y.; Petratos, K.
    Application of the effects of ionic strength reducing agents in the purification and crystallization of chitinase A (2002), Acta Crystallogr. Sect. D, 58, 1593-1596.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.201 ChiA crystallizes both by salting-in and salting-out in the presence of a variety of precipitating agents in a wide range of concentrations and pH values Serratia marcescens

General Stability

EC Number General Stability Organism
3.2.1.201 protein precipitates at concentrations higher than 10 mg/ml in a variety of pH and ionic strength values Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.201 Serratia marcescens O83008
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.201 ChiA binds nonspecifically polysaccharides, which are found in the growth medium. These sugar impurities have to be removed during the purification process Serratia marcescens