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Literature summary extracted from

  • Tokgoz, M.; Inan, K.; Belduz, A.; Gedikli, A.; Canakci, S.
    Cloning, purification, and characterization of a thermophilic ribulokinase from Anoxybacillus kestanbolensis AC26Sari (2014), Turk. J. Biol., 38, 633-639.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.16 2-mercaptoethanol 20 mM, increases ribulokinase activity up to 175% Anoxybacillus kestanbolensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.16 expression in Escherichia coli Anoxybacillus kestanbolensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.16 additional information not inhibitory or activating: K+,Li+, Ca2+, and EDTA Anoxybacillus kestanbolensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.16 0.94
-
 D-ribulose pH 9.0, 60°C Anoxybacillus kestanbolensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.16 Mg2+ 1 mM, increases ribulokinase activity up to 633% Anoxybacillus kestanbolensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.16 61000
-
 x * 61000, SDS-PAGE Anoxybacillus kestanbolensis

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.16 Anoxybacillus kestanbolensis U5JE22
-
-
2.7.1.16 Anoxybacillus kestanbolensis AC26Sari U5JE22
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.16 ATP + D-ribulose
-
 Anoxybacillus kestanbolensis ADP + D-ribulose 5-phosphate
-
 ?
2.7.1.16 ATP + D-ribulose
-
 Anoxybacillus kestanbolensis AC26Sari ADP + D-ribulose 5-phosphate
-
 ?
2.7.1.16 ATP + L-ribulose about 45% of the activity with D-ribulose Anoxybacillus kestanbolensis ADP + L-ribulose 5-phosphate
-
 ?
2.7.1.16 ATP + L-ribulose about 45% of the activity with D-ribulose Anoxybacillus kestanbolensis AC26Sari ADP + L-ribulose 5-phosphate
-
 ?
2.7.1.16 D-xylulose + ATP about 20% of the activity with D-ribulose Anoxybacillus kestanbolensis D-xylulose 5-phosphate + ADP
-
 ?
2.7.1.16 D-xylulose + ATP about 20% of the activity with D-ribulose Anoxybacillus kestanbolensis AC26Sari D-xylulose 5-phosphate + ADP
-
 ?
2.7.1.16 L-xylulose + ATP about 20% of the activity with D-ribulose Anoxybacillus kestanbolensis L-xylulose 5-phosphate + ADP
-
 ?
2.7.1.16 L-xylulose + ATP about 20% of the activity with D-ribulose Anoxybacillus kestanbolensis AC26Sari L-xylulose 5-phosphate + ADP
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.16 ? x * 61000, SDS-PAGE Anoxybacillus kestanbolensis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.16 AraB
-
 Anoxybacillus kestanbolensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.16 60
-
-
 Anoxybacillus kestanbolensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.16 50 75
-
 Anoxybacillus kestanbolensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.16 60
-
 30 min, at least 90% residual activity Anoxybacillus kestanbolensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.16 9
-
-
 Anoxybacillus kestanbolensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.16 5 10
-
 Anoxybacillus kestanbolensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.1.16 10
-
 30 min, 70% residual activity Anoxybacillus kestanbolensis