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Literature summary extracted from

  • Ferriols, V.M.; Yaginuma, R.; Adachi, M.; Takada, K.; Matsunaga, S.; Okada, S.
    Cloning and characterization of farnesyl pyrophosphate synthase from the highly branched isoprenoid producing diatom Rhizosolenia setigera (2015), Sci. Rep., 5, 10246.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.10 expressed in Escherichia coli BL21(DE3) cells Rhizosolenia setigera

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.10 risedronate
-
 Rhizosolenia setigera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.10 0.0106
-
 dimethylallyl diphosphate at pH 7.5% and 30°C Rhizosolenia setigera
2.5.1.10 0.0212
-
 isopentenyl diphosphate with geranyl diphosphate as cosubstrate, at pH 7.5% and 30°C Rhizosolenia setigera
2.5.1.10 0.0215
-
 isopentenyl diphosphate with dimethylallyl diphosphate as cosubstrate, at pH 7.5% and 30°C Rhizosolenia setigera
2.5.1.10 0.063
-
 geranyl diphosphate at pH 7.5% and 30°C Rhizosolenia setigera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.10 53000
-
-
 Rhizosolenia setigera

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate Rhizosolenia setigera
-
 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate Rhizosolenia setigera CCMP1694
-
 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 isopentenyl diphosphate + dimethylallyl diphosphate Rhizosolenia setigera
-
 diphosphate + geranyl diphosphate
-
 ?
2.5.1.10 isopentenyl diphosphate + dimethylallyl diphosphate Rhizosolenia setigera CCMP1694
-
 diphosphate + geranyl diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.10 Rhizosolenia setigera
-
-
-
2.5.1.10 Rhizosolenia setigera CCMP1694
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate
-
 Rhizosolenia setigera diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 geranyl diphosphate + isopentenyl diphosphate
-
 Rhizosolenia setigera CCMP1694 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?
2.5.1.10 isopentenyl diphosphate + dimethylallyl diphosphate
-
 Rhizosolenia setigera diphosphate + geranyl diphosphate
-
 ?
2.5.1.10 isopentenyl diphosphate + dimethylallyl diphosphate
-
 Rhizosolenia setigera CCMP1694 diphosphate + geranyl diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.10 ? x * 48940, calculated from amino acid sequence Rhizosolenia setigera
2.5.1.10 ? x * 53000, recombinant His6-tagged enzyme, SDS-PAGE Rhizosolenia setigera

Synonyms

EC Number Synonyms Comment Organism
2.5.1.10 farnesyl pyrophosphate synthase
-
 Rhizosolenia setigera
2.5.1.10 FPPS
-
 Rhizosolenia setigera

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.10 5.73
-
 dimethylallyl diphosphate at pH 7.5% and 30°C Rhizosolenia setigera
2.5.1.10 6
-
 isopentenyl diphosphate with dimethylallyl diphosphate as cosubstrate, at pH 7.5% and 30°C Rhizosolenia setigera
2.5.1.10 33.5
-
 isopentenyl diphosphate with geranyl diphosphate as cosubstrate, at pH 7.5% and 30°C Rhizosolenia setigera
2.5.1.10 43.5
-
 geranyl diphosphate at pH 7.5% and 30°C Rhizosolenia setigera

General Information

EC Number General Information Comment Organism
2.5.1.10 metabolism the enzyme supplies precursors for highly branched isoprenoid biosynthesis Rhizosolenia setigera