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Literature summary extracted from

  • Wang, Y.; Li, L.; Ma, C.; Gao, C.; Tao, F.; Xu, P.
    Engineering of cofactor regeneration enhances (2S,3S)-2,3-butanediol production from diacetyl (2013), Sci. Rep., 3, 2643.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.76 co-expressed with glucose dehydrogenase and formate dehydrogenase in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.76 26800
-
 x * 26800, SDS-PAGE Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.76 (2S,3S)-butane-2,3-diol + NAD+ Corynebacterium glutamicum
-
 (2S)-acetoin + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.76 Corynebacterium glutamicum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.76 39
-
 at pH 7.4 and 30°C Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.76 (2S)-acetoin + NADH + H+
-
 Corynebacterium glutamicum (2S,3S)-butane-2,3-diol + NAD+
-
 r
1.1.1.76 (2S,3S)-butane-2,3-diol + NAD+
-
 Corynebacterium glutamicum (2S)-acetoin + NADH + H+
-
 r
1.1.1.76 diacetyl + NADH + H+
-
 Corynebacterium glutamicum (2S)-acetoin + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.76 ? x * 26800, SDS-PAGE Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
1.1.1.76 2,3-BDH
-
 Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.76 NAD+
-
 Corynebacterium glutamicum