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Literature summary extracted from

  • Xu, L.; Wu, D.; Liu, L.; Zheng, Q.; Song, Y.; Ye, L.; Sha, S.; Kang, J.; Xin, Y.; Ma, Y.
    Characterization of mycobacterial UDP-N-acetylglucosamine enolpyruvyle transferase (MurA) (2014), Res. Microbiol., 165, 91-101.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.7 expressed in Escherichia coli BL21 cells Mycolicibacterium smegmatis
2.5.1.7 expressed in Escherichia coli BL21 cells Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.7 0.121
-
 phosphoenolpyruvate at pH 8.0 and 37°C Mycobacterium tuberculosis
2.5.1.7 0.199
-
 phosphoenolpyruvate at pH 7.5 and 37°C Mycolicibacterium smegmatis
2.5.1.7 2.32
-
 UDP-N-acetyl-alpha-D-glucosamine at pH 8.0 and 37°C Mycobacterium tuberculosis
2.5.1.7 2.743
-
 UDP-N-acetyl-alpha-D-glucosamine at pH 7.5 and 37°C Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.7 additional information the enzyme is independent of Mg2+ and Ca2+ Mycolicibacterium smegmatis
2.5.1.7 additional information the enzyme is independent of Mg2+ and Ca2+ Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.7 45430
-
 x * 45500, calculated from amino acid sequence Mycolicibacterium smegmatis
2.5.1.7 45500
-
-
 Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine Mycolicibacterium smegmatis
-
 phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
 ?
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine Mycobacterium tuberculosis
-
 phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
 ?
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
 phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
 ?
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine Mycobacterium tuberculosis H37Rv
-
 phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.7 Mycobacterium tuberculosis
-
-
-
2.5.1.7 Mycobacterium tuberculosis H37Rv
-
-
-
2.5.1.7 Mycolicibacterium smegmatis
-
-
-
2.5.1.7 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.7 Ni-NTA column chromatography Mycolicibacterium smegmatis
2.5.1.7 Ni-NTA column chromatography Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine
-
 Mycolicibacterium smegmatis phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
 ?
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine
-
 Mycobacterium tuberculosis phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
 ?
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine
-
 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
 ?
2.5.1.7 phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine
-
 Mycobacterium tuberculosis H37Rv phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.7 ? x * 45500, calculated from amino acid sequence Mycolicibacterium smegmatis
2.5.1.7 ? x * 45430, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.5.1.7 MurA
-
 Mycolicibacterium smegmatis
2.5.1.7 MurA
-
 Mycobacterium tuberculosis
2.5.1.7 UDP-N-acetylglucosamine enolpyruvyle transferase
-
 Mycolicibacterium smegmatis
2.5.1.7 UDP-N-acetylglucosamine enolpyruvyle transferase
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.7 37
-
-
 Mycolicibacterium smegmatis
2.5.1.7 37
-
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.7 0.033
-
 UDP-N-acetyl-alpha-D-glucosamine at pH 7.5 and 37°C Mycolicibacterium smegmatis
2.5.1.7 0.058
-
 phosphoenolpyruvate at pH 7.5 and 37°C Mycolicibacterium smegmatis
2.5.1.7 0.117
-
 phosphoenolpyruvate at pH 8.0 and 37°C Mycobacterium tuberculosis
2.5.1.7 0.147
-
 UDP-N-acetyl-alpha-D-glucosamine at pH 8.0 and 37°C Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.7 7.5
-
-
 Mycolicibacterium smegmatis
2.5.1.7 8
-
-
 Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
2.5.1.7 physiological function murA is an essential gene for growth of Mycobacterium smegmatis Mycolicibacterium smegmatis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.7 0.0119
-
 UDP-N-acetyl-alpha-D-glucosamine at pH 7.5 and 37°C Mycolicibacterium smegmatis
2.5.1.7 0.0636
-
 UDP-N-acetyl-alpha-D-glucosamine at pH 8.0 and 37°C Mycobacterium tuberculosis
2.5.1.7 0.2929
-
 phosphoenolpyruvate at pH 7.5 and 37°C Mycolicibacterium smegmatis
2.5.1.7 0.9636
-
 phosphoenolpyruvate at pH 8.0 and 37°C Mycobacterium tuberculosis