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Literature summary extracted from

  • Hughes, S.J.; Antoshchenko, T.; Kim, K.P.; Smil, D.; Park, H.W.
    Structural characterization of a new N-substituted pantothenamide bound to pantothenate kinases from Klebsiella pneumoniae and Staphylococcus aureus (2014), Proteins, 82, 1542-1548.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.33 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus
2.7.1.33 expressed in Escherichia coli BL21(DE3) cells Klebsiella pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.33 in complex with N-[2-(1,3-benzodioxol-5-yl)ethyl] pantothenamide, hanging drop vapor diffusion method, using 1.4 M sodium citrate, 0.1 M HEPES pH 7.5 and 5% (v/v) ethylene glycol Staphylococcus aureus
2.7.1.33 in complex with N-[2-(1,3-benzodioxol-5-yl)ethyl] pantothenamide, hanging drop vapor diffusion method, using 2 M sodium formate and 0.1 M Tris-HCl pH 8.5 Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate Staphylococcus aureus
-
 ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate Klebsiella pneumoniae
-
 ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate Staphylococcus aureus MSSA476
-
 ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate Klebsiella pneumoniae 342
-
 ADP + (R)-4'-phosphopantothenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.33 Klebsiella pneumoniae B5XYG3
-
-
2.7.1.33 Klebsiella pneumoniae 342 B5XYG3
-
-
2.7.1.33 Staphylococcus aureus Q6G7I0
-
-
2.7.1.33 Staphylococcus aureus MSSA476 Q6G7I0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.33 Ni-NTA resin column chromatography and Superdex 200 gel filtration Staphylococcus aureus
2.7.1.33 Ni-NTA resin column chromatography and Superdex 200 gel filtration Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.33 ATP + (R)-pantothenate
-
 Staphylococcus aureus ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate
-
 Klebsiella pneumoniae ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate
-
 Staphylococcus aureus MSSA476 ADP + (R)-4'-phosphopantothenate
-
 ?
2.7.1.33 ATP + (R)-pantothenate
-
 Klebsiella pneumoniae 342 ADP + (R)-4'-phosphopantothenate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.33 PanK
-
 Staphylococcus aureus
2.7.1.33 PanK
-
 Klebsiella pneumoniae