EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.6.1.17 | D128A | less than 1% of wild-type activity | Escherichia coli |
4.6.1.17 | D17A | about 30% of wild-type activity | Escherichia coli |
4.6.1.17 | E112A | less than 1% of wild-type activity | Escherichia coli |
4.6.1.17 | E114A | less than 1% of wild-type activity | Escherichia coli |
4.6.1.17 | H77A | less than 1% of wild-type activity | Escherichia coli |
4.6.1.17 | K131A | less than 1% of wild-type activity | Escherichia coli |
4.6.1.17 | K21A | about 18% of wild-type activity | Escherichia coli |
4.6.1.17 | K51A | less than 1% of wild-type activity | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.6.1.17 | 0.00021 | - |
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate | His-tagged wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4.6.1.17 | 0.00025 | - |
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4.6.1.17 | 0.001 | - |
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate | mutant K51A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4.6.1.17 | 0.0016 | - |
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate | mutant H77A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4.6.1.17 | 0.0024 | - |
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate | mutant E112A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4.6.1.17 | 0.0043 | - |
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate | mutant E114A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.99.22 | Staphylococcus aureus | P69848 | - |
- |
4.1.99.22 | Staphylococcus aureus NCTC 8325 | P69848 | - |
- |
4.6.1.17 | Escherichia coli | P0A738 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.22 | GTP + S-adenosyl-L-methionine + reduced electron acceptor | - |
Staphylococcus aureus | (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor | pyranopterin triphosphate is formed as a by-product, the amount is 0.43% of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine formed | ? | |
4.1.99.22 | GTP + S-adenosyl-L-methionine + reduced electron acceptor | - |
Staphylococcus aureus NCTC 8325 | (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + oxidized electron acceptor | pyranopterin triphosphate is formed as a by-product, the amount is 0.43% of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine formed | ? | |
4.6.1.17 | (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate | - |
Escherichia coli | cyclic pyranopterin phosphate + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.6.1.17 | cyclic pyranopterin monophosphate synthase accessory protein | - |
Escherichia coli |
4.6.1.17 | MoaC | - |
Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.6.1.17 | 0.00002 | - |
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate | mutant K51A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4.6.1.17 | 0.00004 | - |
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate | mutant E114A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4.6.1.17 | 0.00004 | - |
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate | mutant H77A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4.6.1.17 | 0.00005 | - |
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate | mutant E112A, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4.6.1.17 | 0.0088 | - |
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate | wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
4.6.1.17 | 0.0093 | - |
(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate | His-tagged wild-type, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.99.22 | physiological function | the physiological function of MoaA is the conversion of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate (GTP 3?,8-cyclase), and that of MoaC is to catalyze the rearrangement of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate into cyclic pyranopterin | Staphylococcus aureus |
4.6.1.17 | physiological function | the physiological function of MoaA is the conversion of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate (GTP 3?,8-cyclase), and that of MoaC is to catalyze the rearrangement of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate into cyclic pyranopterin | Escherichia coli |