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Literature summary extracted from

  • Irmer, S.; Podzun, N.; Langel, D.; Heidemann, F.; Kaltenegger, E.; Schemmerling, B.; Geilfus, C.M.; Zoerb, C.; Ober, D.
    New aspect of plant-rhizobia interaction: alkaloid biosynthesis in Crotalaria depends on nodulation (2015), Proc. Natl. Acad. Sci. USA, 112, 4164-4169.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.45 gene hss1, DNA and amino acid sequence determination and analysis, sequence comparissons, quantitative real-time PCR enzyme expression analysis Crotalaria spectabilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.45 spermidine + putrescine Crotalaria spectabilis
-
 sym-homospermidine + propane-1,3-diamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.45 Crotalaria spectabilis A0A0E4G3W1 gene hss1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.45 root nodule
-
 Crotalaria spectabilis
-
2.5.1.45 seedling
-
 Crotalaria spectabilis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.45 spermidine + putrescine
-
 Crotalaria spectabilis sym-homospermidine + propane-1,3-diamine
-
 ?

Expression

EC Number Organism Comment Expression
2.5.1.45 Crotalaria spectabilis exogenously applied nitrate has no impact on polyamine biosynthesis, and enzyme expression additional information
2.5.1.45 Crotalaria spectabilis polymanines in Crotalaria are detectable only when the plants form nodules after infection with their rhizobial partner, nodulation is induced by infection with a Bradyrhizobium strain and triggers the polyamine biosynthesis, leading to induction of the enzyme HSS in nodules up

General Information

EC Number General Information Comment Organism
2.5.1.45 metabolism polymanines in Crotalaria are detectable only when the plants form nodules after infection with their rhizobial partner. But polyamines are synthesized by the plant not by the mcirosymbiont Crotalaria spectabilis
2.5.1.45 additional information exogenously applied nitrate has no impact on polyamine biosynthesis, and enzyme activity, but nodulation is inhibited at higher levels of nitrate by autoregulation Crotalaria spectabilis