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Literature summary extracted from

  • Dailey, H.A.; Gerdes, S.; Dailey, T.A.; Burch, J.S.; Phillips, J.D.
    Noncanonical coproporphyrin-dependent bacterial heme biosynthesis pathway that does not use protoporphyrin (2015), Proc. Natl. Acad. Sci. USA, 112, 2210-2215.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.98.1.1 0.72
-
 protoporphyrin IX pH 7.5, 37°C Mycobacterium tuberculosis
4.99.1.9 0.0018
-
 coproporphyrin III pH 7.5, 37°C Bacillus subtilis
4.99.1.9 0.0105
-
 coproporphyrin III pH 7.5, 37°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.3.15 coproporphyrinogen III + O2 Bacillus subtilis
-
 coproporphyrin III + H2O
-
 ?
1.3.3.15 protoporphyrinogen IX + O2 Bacillus subtilis
-
 protoporphyrin IX + H2O
-
 ?
4.99.1.9 coproporphyrin III + Fe2+ Bacillus subtilis
-
 Fe-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Fe2+ Mycobacterium tuberculosis
-
 Fe-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Fe2+ Cutibacterium acnes
-
 Fe-coproporphyrin III + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.3.15 Bacillus subtilis
-
-
-
4.98.1.1 Mycobacterium tuberculosis
-
-
-
4.99.1.9 Bacillus subtilis
-
-
-
4.99.1.9 Cutibacterium acnes
-
-
-
4.99.1.9 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.98.1.1
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.3.15 coproporphyrinogen III + O2
-
 Bacillus subtilis coproporphyrin III + H2O
-
 ?
1.3.3.15 protoporphyrinogen IX + O2
-
 Bacillus subtilis protoporphyrin IX + H2O
-
 ?
4.98.1.1 protoporphyrin IX + Fe2+
-
 Mycobacterium tuberculosis protoheme IX + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Co2+ specific activity with Co2+ is 2.8fold higher than with Fe2+ Mycobacterium tuberculosis Co-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Co2+ specific activity with Co2+ is 6fold higher than with Fe2+ Bacillus subtilis Co-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Cu2+ specific activity with Cu2+ is 4.15old higher than with Fe2+ Bacillus subtilis Cu-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Cu2+ specific activity with Cu2+ is 5.35old higher than with Fe2+ Mycobacterium tuberculosis Cu-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Fe2+
-
 Bacillus subtilis Fe-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Fe2+
-
 Mycobacterium tuberculosis Fe-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Fe2+
-
 Cutibacterium acnes Fe-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Ni2+ specific activity with Ni2+ is 4fold lower than with Fe2+ Bacillus subtilis Ni-coproporphyrin III + 2 H+
-
 ?
4.99.1.9 coproporphyrin III + Ni2+ specific activity with Ni2+ is 80% compared to activity with Fe2+ Mycobacterium tuberculosis Ni-coproporphyrin III + 2 H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.3.15 coproporphyrinogen oxidase
-
 Bacillus subtilis
1.3.3.15 hemY
-
 Bacillus subtilis
1.3.3.15 protoporphyrinogen oxidase
-
 Bacillus subtilis
4.98.1.1 hemH
-
 Mycobacterium tuberculosis
4.99.1.9 hemH
-
 Bacillus subtilis
4.99.1.9 hemH
-
 Mycobacterium tuberculosis
4.99.1.9 hemH gene name. HemH and HemQ exist as a fusion protein Cutibacterium acnes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.98.1.1 0.013
-
 protoporphyrin IX pH 7.5, 37°C Mycobacterium tuberculosis
4.99.1.9 0.0078
-
 coproporphyrin III pH 7.5, 37°C Bacillus subtilis
4.99.1.9 0.03
-
 coproporphyrin III pH 7.5, 37°C Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.98.1.1 0.018
-
 protoporphyrin IX pH 7.5, 37°C Mycobacterium tuberculosis
4.99.1.9 2.9
-
 coproporphyrin III pH 7.5, 37°C Mycobacterium tuberculosis
4.99.1.9 4.3
-
 coproporphyrin III pH 7.5, 37°C Bacillus subtilis