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Literature summary extracted from

  • Rosado, L.A.; Vasconcelos, I.B.; Palma, M.S.; Frappier, V.; Najmanovich, R.J.; Santos, D.S.; Basso, L.A.
    The mode of action of recombinant Mycobacterium tuberculosis shikimate kinase: kinetics and thermodynamics analyses (2013), PLoS ONE, 8, e61918.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.71 expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.71 0.112
-
 ATP at pH 7.6 and 25°C Mycobacterium tuberculosis
2.7.1.71 0.65
-
 shikimate at pH 7.6 and 25°C Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.71 Mg2+ required Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.71 18451
-
-
 Mycobacterium tuberculosis
2.7.1.71 20700
-
 gel filtration Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.71 ATP + shikimate Mycobacterium tuberculosis
-
 ADP + 3-phosphoshikimate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.71 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.71 phenyl Sepharose column chromatography and Sephacryl S-100 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.71 ATP + shikimate
-
 Mycobacterium tuberculosis ADP + 3-phosphoshikimate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.1.71 monomer 1 * 18451, calculated from amino acid sequence Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.71 AroL
-
 Mycobacterium tuberculosis
2.7.1.71 type II shikimate kinase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.71 60
-
 ATP at pH 7.6 and 25°C Mycobacterium tuberculosis
2.7.1.71 60
-
 shikimate at pH 7.6 and 25°C Mycobacterium tuberculosis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.71 54
-
 ATP at pH 7.6 and 25°C Mycobacterium tuberculosis
2.7.1.71 90
-
 shikimate at pH 7.6 and 25°C Mycobacterium tuberculosis