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Literature summary extracted from

  • Yu, J.; Haldar, M.; Mallik, S.; Srivastava, D.K.
    Role of the substrate specificity-defining residues of human SIRT5 in modulating the structural stability and inhibitory features of the enzyme (2016), PLoS ONE, 11, e0152467.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.B43 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.B43 R105L the mutant enzyme favores the deacetylase reaction over the desuccinylation reaction Homo sapiens
2.3.1.B43 Y102A the mutant enzyme catalyzes both deacetylation and desuccinylation reactions with comparable efficiencies Homo sapiens
2.3.1.B43 Y102A/R105I the mutant enzyme favores the deacetylase reaction over the desuccinylation reaction Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.B43 Isonicotinamide
-
Homo sapiens
2.3.1.B43 additional information not inhibited by EX527 (i.e. 6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide) Homo sapiens
2.3.1.B43 nicotinamide competitive inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.B43 0.069
-
NAD+ wild type enzyme, with [protein]-N6-succinyl-L-lysine as cosubstrate, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 0.29
-
[protein]-N6-succinyl-L-lysine wild type enzyme, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 0.321
-
NAD+ mutant enzyme Y102A, with [protein]-N6-succinyl-L-lysine as cosubstrate, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 0.388
-
[protein]-N6-succinyl-L-lysine mutant enzyme Y102A/R105I, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 0.609
-
NAD+ mutant enzyme R105I, with [protein]-N6-acetyl-L-lysine as cosubstrate, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 0.72
-
[protein]-N6-acetyl-L-lysine mutant enzyme Y102A, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 1.194
-
NAD+ mutant enzyme Y102A/R105I, with [protein]-N6-acetyl-L-lysine as cosubstrate, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 1.605
-
NAD+ mutant enzyme Y102A, with [protein]-N6-acetyl-L-lysine as cosubstrate, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 1.658
-
[protein]-N6-succinyl-L-lysine mutant enzyme R105I, at 8.0 pH and 25°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.B43 NAD+ + [protein]-N6-acetyl-L-lysine Homo sapiens weak deacetylase activity nicotinamide + [protein]-L-lysine + 2'-O-acetyl-ADP-ribose
-
?
2.3.1.B43 NAD+ + [protein]-N6-succinyl-L-lysine Homo sapiens strong succinylase activity nicotinamide + [protein]-L-lysine + 2'-O-succinyl-ADP-ribose
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.B43 Homo sapiens Q9NXA8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.B43 HisTrap column chromatography and Hitrap column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.B43 NAD+ + Ac-Suc-L-Lys-7-amido-4-methylcoumarin
-
Homo sapiens nicotinamide + ?
-
?
2.3.1.B43 NAD+ + Fluor-de-Lys
-
Homo sapiens nicotinamide + ?
-
?
2.3.1.B43 NAD+ + [protein]-N6-acetyl-L-lysine weak deacetylase activity Homo sapiens nicotinamide + [protein]-L-lysine + 2'-O-acetyl-ADP-ribose
-
?
2.3.1.B43 NAD+ + [protein]-N6-succinyl-L-lysine strong succinylase activity Homo sapiens nicotinamide + [protein]-L-lysine + 2'-O-succinyl-ADP-ribose
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.B43 SIRT5
-
Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.B43 52.9
-
melting temperature Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.B43 0.000026
-
[protein]-N6-succinyl-L-lysine mutant enzyme Y102A/R105I, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 0.000026
-
NAD+ mutant enzyme Y102A/R105I, with [protein]-N6-acetyl-L-lysine as cosubstrate, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 0.000066
-
[protein]-N6-acetyl-L-lysine mutant enzyme Y102A, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 0.000122
-
NAD+ mutant enzyme R105I, with [protein]-N6-acetyl-L-lysine as cosubstrate, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 0.000274
-
[protein]-N6-succinyl-L-lysine mutant enzyme R105I, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 0.000274
-
NAD+ mutant enzyme Y102A, with [protein]-N6-succinyl-L-lysine as cosubstrate, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 0.04
-
[protein]-N6-succinyl-L-lysine wild type enzyme, at 8.0 pH and 25°C Homo sapiens
2.3.1.B43 0.04
-
NAD+ wild type enzyme, with [protein]-N6-succinyl-L-lysine as cosubstrate, at 8.0 pH and 25°C Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.B43 0.0175
-
nicotinamide wild type enzyme, at pH 8.0 and 25°C Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.B43 0.0111
-
wild type enzyme, with 0.05 mM NAD+, at 8.0 pH and 25°C Homo sapiens Isonicotinamide
2.3.1.B43 0.0347
-
wild type enzyme, at 8.0 pH and 25°C Homo sapiens Isonicotinamide