EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.27 | expressed in Escherichia coli Top10 cells | Nostoc sp. |
2.5.1.27 | gene NoIPT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) STAR | Nostoc sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.27 | additional information | - |
additional information | Michaelis-Menten kinetics | Nostoc sp. | |
2.5.1.27 | 0.0063 | - |
AMP | at pH 7.5 and 25°C | Nostoc sp. | |
2.5.1.27 | 0.02 | - |
dimethylallyl diphosphate | pH 7.5, 25°C, recombinant enzyme | Nostoc sp. | |
2.5.1.27 | 0.02705 | - |
dimethylallyl diphosphate | at pH 7.5 and 25°C | Nostoc sp. | |
2.5.1.27 | 0.15 | - |
AMP | pH 7.5, 25°C, recombinant enzyme | Nostoc sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.27 | Mg2+ | required | Nostoc sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.5.1.27 | 27900 | - |
- |
Nostoc sp. |
2.5.1.27 | 28300 | - |
- |
Nostoc sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.27 | dimethylallyl diphosphate + AMP | Nostoc sp. | - |
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate | - |
? | |
2.5.1.27 | dimethylallyl diphosphate + AMP | Nostoc sp. PCC 7120 | - |
diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.27 | Nostoc sp. | - |
- |
- |
2.5.1.27 | Nostoc sp. | Q8Z078 | gene NoIPT1 | - |
2.5.1.27 | Nostoc sp. PCC 7120 | - |
- |
- |
2.5.1.27 | Nostoc sp. PCC 7120 | Q8Z078 | gene NoIPT1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.27 | Ni-NTA column chromatography | Nostoc sp. |
2.5.1.27 | recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) STAR by anion exchange and nickel affinity chromatography to apparent homogeneity | Nostoc sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.27 | 0.01122 | - |
purified recombinant enzyme, pH 7.5, 25°C | Nostoc sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.27 | (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate + AMP | HMBPP, the enzyme uses the hydroxylated side chain precursor to form trans-zeatin-5'-monophosphate in the reaction with AMP, reaction rate is 0.5% of the rate with dimethylallyl díphosphate | Nostoc sp. | diphosphate + trans-zeatin riboside 5'phosphate | - |
? | |
2.5.1.27 | (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate + AMP | HMBPP, the enzyme uses the hydroxylated side chain precursor to form trans-zeatin-5'-monophosphate in the reaction with AMP, reaction rate is 0.5% of the rate with dimethylallyl díphosphate | Nostoc sp. PCC 7120 | diphosphate + trans-zeatin riboside 5'phosphate | - |
? | |
2.5.1.27 | dimethylallyl diphosphate + AMP | - |
Nostoc sp. | diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate | - |
? | |
2.5.1.27 | dimethylallyl diphosphate + AMP | - |
Nostoc sp. PCC 7120 | diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate | - |
? | |
2.5.1.27 | additional information | less than 0.5% activity with (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate plus AMP | Nostoc sp. | ? | - |
? | |
2.5.1.27 | additional information | no activity with ADP and ATP. Isopentenyl diphosphate, an isomer of DMAPP, is no substrate with either of the side chain acceptor substrates. The enzyme does not show tRNA IPT activity, as it does not prenylate synthetic tRNA-stem-loop minihelix substrate | Nostoc sp. | ? | - |
? | |
2.5.1.27 | additional information | the enzyme shows no activity with ATP and ADP and no tRNA isopentenyl transferase activity | Nostoc sp. | ? | - |
? | |
2.5.1.27 | additional information | less than 0.5% activity with (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate plus AMP | Nostoc sp. PCC 7120 | ? | - |
? | |
2.5.1.27 | additional information | the enzyme shows no activity with ATP and ADP and no tRNA isopentenyl transferase activity | Nostoc sp. PCC 7120 | ? | - |
? | |
2.5.1.27 | additional information | no activity with ADP and ATP. Isopentenyl diphosphate, an isomer of DMAPP, is no substrate with either of the side chain acceptor substrates. The enzyme does not show tRNA IPT activity, as it does not prenylate synthetic tRNA-stem-loop minihelix substrate | Nostoc sp. PCC 7120 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.5.1.27 | ? | x * 27900, recombinant enzyme, SDS-PAGE, x * 28300, about, sequence calculation | Nostoc sp. |
2.5.1.27 | ? | x * 27900, SDS-PAGE | Nostoc sp. |
2.5.1.27 | ? | x * 28300, calculated from amino acid sequence | Nostoc sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.27 | adenylate dimethylallyltransferase | - |
Nostoc sp. |
2.5.1.27 | adenylate IPT | - |
Nostoc sp. |
2.5.1.27 | adenylate isopentenyl transferase | - |
Nostoc sp. |
2.5.1.27 | adenylate-type isopentenyl transferase | - |
Nostoc sp. |
2.5.1.27 | IPT1 | - |
Nostoc sp. |
2.5.1.27 | isopentenyl transferase | - |
Nostoc sp. |
2.5.1.27 | NoIPT1 | - |
Nostoc sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.27 | 25 | - |
assay at | Nostoc sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.27 | 7.5 | - |
- |
Nostoc sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.5.1.27 | evolution | adenylate-type isopentenyl transferases might have evolved several times independently, phylogenetic analysis. Key enzymes for cytokinin synthesis and degradation (adenylate IPT and CKX) are found only in few species of cyanobacteria | Nostoc sp. |
2.5.1.27 | metabolism | the biosynthesis of cytokinins starts with a transfer of dimethylallyl chain from dimethylallyl pyrophosphate (DMAPP) or (E)-4-hydroxy-3-methyl-but-2-enyl diphosphate (HMBPP) to N6-amino group of free or tRNA-bound adenosine phosphates. The reactions are catalyzed by related but distinct enzymes, adenylate dimethylallyltransferase (EC 2.5.1.27) and tRNA dimethylallyltransferase (EC 2.5.1.75), respectively, more frequently termed as isopentenyl transferases | Nostoc sp. |