Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wang, L.; Jiang, Y.L.; Zhang, J.R.; Zhou, C.Z.; Chen, Y.
    Structural and enzymatic characterization of the choline kinase LicA from Streptococcus pneumoniae (2015), PLoS ONE, 10, e0120467.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.32 expressed in Escherichia coli BL21(DE3) cells Streptococcus pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.32 native and selenomethionine-labelled enzyme, hanging drop vapor diffusion method, using 0.1M HEPES, pH 7.5, 1.2 M sodium citrate and 4% (v/v) glycerol Streptococcus pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.32 D167A the mutant shows about 14% activity compared to the wild type enzyme Streptococcus pneumoniae
2.7.1.32 T29A the mutant shows about 10% activity compared to the wild type enzyme Streptococcus pneumoniae
2.7.1.32 T29S the mutant shows slightly increased activity compared to the wild type enzyme Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.32 EDTA about 10% residual activity at 5 mM Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.32 0.15
-
 choline at pH 8.0 and 37°C Streptococcus pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.32 cytoplasm
-
 Streptococcus pneumoniae 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.32 Mg2+ dependent on Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.32 ATP + choline Streptococcus pneumoniae
-
 ADP + phosphocholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.32 Streptococcus pneumoniae Q8DPI4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.32 Ni-NTA column chromatography and Superdex 200 gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.32 ATP + choline
-
 Streptococcus pneumoniae ADP + phosphocholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.32 LicA
-
 Streptococcus pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.32 5.1
-
 choline at pH 8.0 and 37°C Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.32 8
-
-
 Streptococcus pneumoniae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.32 33.3
-
 choline at pH 8.0 and 37°C Streptococcus pneumoniae