Literature summary extracted from

Buanafina, M.M.; Dalton, S.; Langdon, T.; Timms-Taravella, E.; Shearer, E.A.; Morris, P.
Functional co-expression of a fungal ferulic acid esterase and a beta-1,4 endoxylanase in Festuca arundinacea (tall fescue) modifies post-harvest cell wall deconstruction (2015), Planta, 242, 97-111.

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.1.73	Aspergillus niger	-	-	-
3.2.1.8	Trichoderma reesei	-	-	-

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.1.73	FAEA	-	Aspergillus niger
3.2.1.8	Xyn2	-	Trichoderma reesei

General Information

EC Number	General Information	Comment	Organism
3.1.1.73	physiological function	coexpression of vacuole- or apoplast-targeted ferulic acid esterase from Aspergillus niger and senescence-induced and apoplast-targeted beta-1,4 endo-xylanase from Trichoderma reesei in Festuca arundinacea. Xylanase activity in senescent leaves increases and ferulic acid esterase activity decreases after tillering. Plants coexpressing both enzymes in the apoplast, with the lowest levels of ferulate monomers and dimers and the lowest levels of cell wall arabinoxylans, release ten times more cell wall hydroxycinnamic acids and five times more arabinoxylan from the cell wall on autodigestion compared to expression of ferulic acid esterase or xylanase alone. These plants also show a 31% increase in cellulase-mediated release of reducing sugars, a 5% point increase in in vitro dry matter digestibility and a 23% increase in acetyl bromide-soluble lignin. Plant growth is adversely affected by expressing ferulic acid esterase in the apoplast, giving plants with narrower shorted leaves, and a 71% decrease in biomass	Aspergillus niger
3.2.1.8	physiological function	coexpression of vacuole- or apoplast-targeted ferulic acid esterase from Aspergillus niger and senescence-induced and apoplast-targeted beta-1,4 endo-xylanase from Trichoderma reesei in Festuca arundinacea. Xylanase activity in senescent leaves increases and ferulic acid esterase activity decreases after tillering. Plants coexpressing both enzymes in the apoplast, with the lowest levels of ferulate monomers and dimers and the lowest levels of cell wall arabinoxylans, release ten times more cell wall hydroxycinnamic acids and five times more arabinoxylan from the cell wall on autodigestion compared to expression of ferulic acid esterase or xylanase alone. These plants also show a 31% increase in cellulase-mediated release of reducing sugars, a 5% point increase in in vitro dry matter digestibility and a 23% increase in acetyl bromide-soluble lignin	Trichoderma reesei

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Release 2023.1 (January 2023)