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Literature summary extracted from

  • Muramoto, T.; Tsurui, N.; Terry, M.; Yokota, A.; Kohchi, T.
    Expression and biochemical properties of a ferredoxin-dependent heme oxygenase required for phytochrome chromophore synthesis (2002), Plant Physiol., 130, 1958-1966.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.15.20 4,5-dihydroxy-1,3-benzene disulfonic acid
-
 Arabidopsis thaliana
1.14.15.20 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid significantly increases enzyme activity Arabidopsis thaliana
1.14.15.20 dehydroascorbate
-
 Arabidopsis thaliana
1.14.15.20 desferrioxamine
-
 Arabidopsis thaliana
1.14.15.20 isoascorbate
-
 Arabidopsis thaliana
1.14.15.20 L-ascorbate the enzyme requires an iron chelator and second reductant, such as L-ascorbate, for full activity Arabidopsis thaliana
1.14.15.20 o-phenanthroline
-
 Arabidopsis thaliana
1.14.15.20 Trolox
-
 Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.20 expressed in Escherichia coli BL21 cells Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.20 0.0013
-
 hemin at pH 7.2 and 25°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.15.20 27000
-
 x * 27000, recombinant enzyme, SDS-PAGE Arabidopsis thaliana
1.14.15.20 53000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.20 hemin + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Arabidopsis thaliana
-
 biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.20 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.20 Q sepharose column chromatography Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
1.14.15.20 -30°C, in 50 mM HEPES-NaOH (pH 7.2) with 40% (v/v) glycerol, at least 3 months, no loss of activity Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.20 hemin + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Arabidopsis thaliana biliverdin IXalpha + Fe2+ + CO + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.15.20 ? x * 27000, recombinant enzyme, SDS-PAGE Arabidopsis thaliana
1.14.15.20 ? x * 53000, GST-tagged enzyme, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.14.15.20 ferredoxin-dependent heme oxygenase
-
 Arabidopsis thaliana
1.14.15.20 Ho1
-
 Arabidopsis thaliana
1.14.15.20 HY1
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.15.20 50
-
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.15.20 7.2
-
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.15.20 heme the enzyme forms a 1:1 complex with heme Arabidopsis thaliana