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Literature summary extracted from

  • Kammerer, L.; De-Eknamkul, W.; Zenk, M.H.
    Enzymic 12-hydroxylation and 12-O-methylation of dihydrochelirubine in dihydromacarpine formation by Thalictrum bulgaricum (1994), Phytochemistry, 36, 1409-1416.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.14.101 2-Methyl-1,2-di(3-pyridyl)-1-propanone i.e. methyrapone Thalictrum bulgaricum
1.14.14.101 5-hydroxy-1,4-naphthoquinone i.e. juglone Thalictrum bulgaricum
1.14.14.101 CO inhibition in the dark, effect is partly reversible upon illumination Thalictrum bulgaricum
1.14.14.101 cytochrome c
-
Thalictrum bulgaricum
1.14.14.101 ketoconazole
-
Thalictrum bulgaricum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.101 microsome associated with Thalictrum bulgaricum
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.101 dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2 Thalictrum bulgaricum involved the penultimate step in the biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid found in nature 12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
2.1.1.120 S-adenosyl-L-methionine + 12-hydroxydihydrochelirubine Thalictrum bulgaricum involved in biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid, not significantly affected by elicitation ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.101 Thalictrum bulgaricum
-
-
-
2.1.1.120 Thalictrum bulgaricum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.120
-
Thalictrum bulgaricum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.101 cell culture yeast-elicited cells Thalictrum bulgaricum
-
2.1.1.120 cell culture
-
Thalictrum bulgaricum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.101 dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2
-
Thalictrum bulgaricum 12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
1.14.14.101 dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2 involved the penultimate step in the biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid found in nature Thalictrum bulgaricum 12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
?
2.1.1.120 S-adenosyl-L-methionine + 12-hydroxydihydrochelirubine
-
Thalictrum bulgaricum S-adenosyl-L-homocysteine + dihydromacarpine
-
?
2.1.1.120 S-adenosyl-L-methionine + 12-hydroxydihydrochelirubine involved in biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid, not significantly affected by elicitation Thalictrum bulgaricum ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.101 30
-
-
Thalictrum bulgaricum
2.1.1.120 30
-
-
Thalictrum bulgaricum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.101 8.5
-
-
Thalictrum bulgaricum
2.1.1.120 7.5
-
-
Thalictrum bulgaricum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.101 cytochrome P450 dependent on Thalictrum bulgaricum