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Literature summary extracted from

  • Kaltenegger, E.; Eich, E.; Ober, D.
    Evolution of homospermidine synthase in the convolvulaceae: a story of gene duplication, gene loss, and periods of various selection pressures (2013), Plant Cell, 25, 1213-1227.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.45 gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli Ipomoea neei
2.5.1.45 gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli Ipomoea hederifolia
2.5.1.45 gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli Ipomoea alba
2.5.1.45 gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli Ipomoea meyeri
2.5.1.45 gene hss, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme in Escherichia coli Distimake quinquefolius
2.5.1.46 gene dhs, single-copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, analysis of gene duplications in Convolvulaceae species Convolvulus arvensis
2.5.1.46 gene dhs, single-copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, analysis of gene duplications in Convolvulaceae species Ipomoea neei
2.5.1.46 gene dhs, single-copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, analysis of gene duplications in Convolvulaceae species Ipomoea hederifolia
2.5.1.46 gene dhs, single-copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, analysis of gene duplications in Convolvulaceae species Ipomoea alba
2.5.1.46 gene dhs, single-copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, analysis of gene duplications in Convolvulaceae species Ipomoea meyeri
2.5.1.46 gene dhs, single-copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, analysis of gene duplications in Convolvulaceae species Distimake quinquefolius
2.5.1.46 gene dhs, single-copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, analysis of gene duplications in Convolvulaceae species Convolvulus tricolor

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.45 I277D site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and slightly reduced deoxyhypusine synthase activity compared to the wild-type Ipomoea neei
2.5.1.45 I277D/N281D site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type Ipomoea neei
2.5.1.45 N266H site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type Ipomoea neei
2.5.1.45 N266H/I277D site-directed mutagenesis, the mutant shows increased HSS activity with putrescine, but unaltered deoxyhypusine synthase activity compared to the wild-type Ipomoea neei
2.5.1.45 N266H/I277D/N281D site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type Ipomoea neei
2.5.1.45 N266H/N281D site-directed mutagenesis, the mutant shows increased HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type Ipomoea neei
2.5.1.45 N281D site-directed mutagenesis, the mutant shows slightly reduced HSS activity with putrescine and reduced deoxyhypusine synthase activity compared to the wild-type Ipomoea neei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.45 41300
-
-
 Distimake quinquefolius
2.5.1.45 41500
-
-
 Ipomoea alba
2.5.1.45 41500
-
-
 Ipomoea meyeri
2.5.1.45 41600
-
 x * 41600, about, sequence calculation Ipomoea neei
2.5.1.45 41600
-
 x * 41600, about, sequence calculation Ipomoea hederifolia
2.5.1.46 42100
-
-
 Distimake quinquefolius
2.5.1.46 42200
-
-
 Ipomoea neei
2.5.1.46 42200
-
-
 Ipomoea meyeri
2.5.1.46 42300
-
-
 Ipomoea hederifolia
2.5.1.46 42300
-
-
 Ipomoea alba

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.45 putrescine + spermidine Ipomoea neei
-
 sym-homospermidine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermidine Ipomoea hederifolia
-
 sym-homospermidine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermidine Ipomoea alba
-
 sym-homospermidine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermidine Ipomoea meyeri
-
 sym-homospermidine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermidine Distimake quinquefolius
-
 sym-homospermidine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Convolvulus arvensis
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Ipomoea neei
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Ipomoea hederifolia
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Ipomoea alba
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Ipomoea meyeri
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Distimake quinquefolius
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine Convolvulus tricolor
-
 [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.45 Distimake quinquefolius
-
 gene hss
-
2.5.1.45 Ipomoea alba
-
 gene hss
-
2.5.1.45 Ipomoea hederifolia
-
 gene hss
-
2.5.1.45 Ipomoea meyeri
-
 gene hss
-
2.5.1.45 Ipomoea neei
-
 gene hss
-
2.5.1.46 Convolvulus arvensis
-
 gene dhs
-
2.5.1.46 Convolvulus tricolor
-
 gene dhs
-
2.5.1.46 Distimake quinquefolius
-
 gene dhs
-
2.5.1.46 Ipomoea alba
-
 gene dhs
-
2.5.1.46 Ipomoea hederifolia
-
 gene dhs
-
2.5.1.46 Ipomoea meyeri
-
 gene dhs
-
2.5.1.46 Ipomoea neei
-
 gene dhs
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.45 recombinant tagged enzyme from Escherichia coli by affinity chromatography Ipomoea neei
2.5.1.45 recombinant tagged enzyme from Escherichia coli by affinity chromatography Ipomoea hederifolia
2.5.1.45 recombinant tagged enzyme from Escherichia coli by affinity chromatography Ipomoea alba
2.5.1.45 recombinant tagged enzyme from Escherichia coli by affinity chromatography Ipomoea meyeri
2.5.1.45 recombinant tagged enzyme from Escherichia coli by affinity chromatography Distimake quinquefolius

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.45 additional information tissue-specific expression of enzyme HSS Ipomoea neei
-
2.5.1.45 additional information tissue-specific expression of enzyme HSS Ipomoea hederifolia
-
2.5.1.45 additional information tissue-specific expression of enzyme HSS Ipomoea alba
-
2.5.1.45 additional information tissue-specific expression of enzyme HSS Ipomoea meyeri
-
2.5.1.45 additional information tissue-specific expression of enzyme HSS Distimake quinquefolius
-
2.5.1.45 root
-
 Ipomoea neei
-
2.5.1.45 root
-
 Ipomoea hederifolia
-
2.5.1.45 root
-
 Ipomoea alba
-
2.5.1.45 root
-
 Ipomoea meyeri
-
2.5.1.45 root
-
 Distimake quinquefolius
-
2.5.1.45 shoot young Ipomoea neei
-
2.5.1.45 shoot young Ipomoea hederifolia
-
2.5.1.45 shoot young Ipomoea alba
-
2.5.1.45 shoot young Ipomoea meyeri
-
2.5.1.45 shoot young Distimake quinquefolius
-
2.5.1.46 additional information tissue-specific expression of enzyme DSS Ipomoea neei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.45 0.047
-
 purified recombinant enzyme, pH and temperature not specified in the publication Ipomoea alba
2.5.1.45 0.062
-
 purified recombinant enzyme, pH and temperature not specified in the publication Ipomoea neei
2.5.1.45 0.136
-
 purified recombinant enzyme, pH and temperature not specified in the publication Ipomoea hederifolia
2.5.1.45 0.138
-
 purified recombinant enzyme, pH and temperature not specified in the publication Ipomoea meyeri
2.5.1.45 0.138
-
 purified recombinant enzyme, pH and temperature not specified in the publication Distimake quinquefolius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.45 putrescine + spermidine
-
 Ipomoea neei sym-homospermidine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermidine
-
 Ipomoea hederifolia sym-homospermidine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermidine
-
 Ipomoea alba sym-homospermidine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermidine
-
 Ipomoea meyeri sym-homospermidine + propane-1,3-diamine
-
 ?
2.5.1.45 putrescine + spermidine
-
 Distimake quinquefolius sym-homospermidine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Convolvulus arvensis [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Ipomoea neei [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Ipomoea hederifolia [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Ipomoea alba [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Ipomoea meyeri [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Distimake quinquefolius [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?
2.5.1.46 [eIF5A-precursor]-lysine + spermidine
-
 Convolvulus tricolor [eIF5A-precursor]-deoxyhypusine + propane-1,3-diamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.45 ? x * 41600, about, sequence calculation Ipomoea neei
2.5.1.45 ? x * 41600, about, sequence calculation Ipomoea hederifolia
2.5.1.45 ? x * 41300, about, sequence calculation Distimake quinquefolius
2.5.1.45 ? x * 41500, about, sequence calculation Ipomoea alba
2.5.1.45 ? x * 41500, about, sequence calculation Ipomoea meyeri
2.5.1.46 ? x * 42100, about, sequence calculation Distimake quinquefolius
2.5.1.46 ? x * 42200, about, sequence calculation Ipomoea neei
2.5.1.46 ? x * 42200, about, sequence calculation Ipomoea meyeri
2.5.1.46 ? x * 42300, about, sequence calculation Ipomoea hederifolia
2.5.1.46 ? x * 42300, about, sequence calculation Ipomoea alba

Synonyms

EC Number Synonyms Comment Organism
2.5.1.45 HSS
-
 Ipomoea neei
2.5.1.45 HSS
-
 Ipomoea hederifolia
2.5.1.45 HSS
-
 Ipomoea alba
2.5.1.45 HSS
-
 Ipomoea meyeri
2.5.1.45 HSS
-
 Distimake quinquefolius
2.5.1.46 DHS
-
 Convolvulus arvensis
2.5.1.46 DHS
-
 Ipomoea neei
2.5.1.46 DHS
-
 Ipomoea hederifolia
2.5.1.46 DHS
-
 Ipomoea alba
2.5.1.46 DHS
-
 Ipomoea meyeri
2.5.1.46 DHS
-
 Distimake quinquefolius
2.5.1.46 DHS
-
 Convolvulus tricolor

General Information

EC Number General Information Comment Organism
2.5.1.45 evolution the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection Ipomoea neei
2.5.1.45 evolution the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection Ipomoea hederifolia
2.5.1.45 evolution the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection Ipomoea alba
2.5.1.45 evolution the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection Ipomoea meyeri
2.5.1.45 evolution the gene encoding enzyme HSS origins from a single duplication event of a single-copy gene encoding deoxyhypusine synthase, DHS, EC 2.5.1.46. This duplication event was followed by several losses of a functional gene copy attributable to gene loss or pseudogenization. Statistical analyses of sequence data suggest that, in those lineages in which the gene copy was successfully recruited as HSS, the gene duplication event was followed by phases of various selection pressures, including purifying selection, relaxed functional constraints, and possibly positive Darwinian selection Distimake quinquefolius
2.5.1.45 metabolism homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis Ipomoea neei
2.5.1.45 metabolism homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis Ipomoea hederifolia
2.5.1.45 metabolism homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis Ipomoea alba
2.5.1.45 metabolism homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis Ipomoea meyeri
2.5.1.45 metabolism homospermidine synthase is the first pathway-specific enzyme of pyrrolizidine alkaloid biosynthesis Distimake quinquefolius
2.5.1.45 additional information the Convulvulaceae species Ipomoea alba contains ipanguline-type polyamines. Detection of sites that are involved in optimizing HSS functionality Ipomoea alba
2.5.1.45 additional information the Convulvulaceae species Ipomoea hederifolia contains ipanguline-type polyamines. Detection of sites that are involved in optimizing HSS functionality Ipomoea hederifolia
2.5.1.45 additional information the Convulvulaceae species Ipomoea meyeri contains ipanguline- and triangularine-type polyamines. Detection of sites that are involved in optimizing HSS functionality Ipomoea meyeri
2.5.1.45 additional information the Convulvulaceae species Ipomoea neei contains ipanguline-type polyamines. Detection of sites that are involved in optimizing HSS functionality Ipomoea neei
2.5.1.45 additional information the Convulvulaceae species Merremia quinquefolia contains ipanguline- and triangularine-type polyamines. Detection of sites that are involved in optimizing HSS functionality Distimake quinquefolius
2.5.1.46 evolution homospermidine synthase, HSS, EC 2.5.1.44/45, evolved from the gene encoding deoxyhypusine synthase, DHS, by gene duplication. HSS and deoxyhypusine synthase have also diverged with respect to their regulation Convolvulus arvensis
2.5.1.46 evolution homospermidine synthase, HSS, EC 2.5.1.44/45, evolved from the gene encoding deoxyhypusine synthase, DHS, by gene duplication. HSS and deoxyhypusine synthase have also diverged with respect to their regulation Ipomoea neei
2.5.1.46 evolution homospermidine synthase, HSS, EC 2.5.1.44/45, evolved from the gene encoding deoxyhypusine synthase, DHS, by gene duplication. HSS and deoxyhypusine synthase have also diverged with respect to their regulation Ipomoea hederifolia
2.5.1.46 evolution homospermidine synthase, HSS, EC 2.5.1.44/45, evolved from the gene encoding deoxyhypusine synthase, DHS, by gene duplication. HSS and deoxyhypusine synthase have also diverged with respect to their regulation Ipomoea alba
2.5.1.46 evolution homospermidine synthase, HSS, EC 2.5.1.44/45, evolved from the gene encoding deoxyhypusine synthase, DHS, by gene duplication. HSS and deoxyhypusine synthase have also diverged with respect to their regulation Ipomoea meyeri
2.5.1.46 evolution homospermidine synthase, HSS, EC 2.5.1.44/45, evolved from the gene encoding deoxyhypusine synthase, DHS, by gene duplication. HSS and deoxyhypusine synthase have also diverged with respect to their regulation Distimake quinquefolius
2.5.1.46 evolution homospermidine synthase, HSS, EC 2.5.1.44/45, evolved from the gene encoding deoxyhypusine synthase, DHS, by gene duplication. HSS and deoxyhypusine synthase have also diverged with respect to their regulation Convolvulus tricolor
2.5.1.46 additional information the Convulvulaceae species Ipomoea hederifolia contains ipanguline- and triangularine-type polyamines Ipomoea hederifolia
2.5.1.46 additional information the Convulvulaceae species Ipomoea meyeri contains triangularine-type polyamines Ipomoea meyeri
2.5.1.46 additional information the Convulvulaceae species Ipomoea neei contains ipanguline-type polyamines Ipomoea neei
2.5.1.46 additional information the Convulvulaceae species Merremia quinquefolia contains lycopsamine-type polyamines Distimake quinquefolius