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Literature summary extracted from

  • Neumann, P.; Lakomek, K.; Naumann, P.T.; Erwin, W.M.; Lauhon, C.T.; Ficner, R.
    Crystal structure of a 4-thiouridine synthetase-RNA complex reveals specificity of tRNA U8 modification (2014), Nucleic Acids Res., 42, 6673-6685.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.B3 expressed in Escherichia coli Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.1.B3 in complex with a truncated tRNA, sitting drop vapor diffusion method, using 2 M sodium formate, 100 mM sodium citrate, pH 4.6, 2 mM dithiothreitol Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.B3 sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8) Thermotoga maritima
-
 sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
-
 ?
2.8.1.B3 sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8) Thermotoga maritima ATCC 43589
-
 sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.B3 Thermotoga maritima Q9X220
-
-
2.8.1.B3 Thermotoga maritima ATCC 43589 Q9X220
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.B3
-
 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.B3 sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8)
-
 Thermotoga maritima sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
-
 ?
2.8.1.B3 sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8)
-
 Thermotoga maritima ATCC 43589 sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.8.1.B3 4-thiouridine synthetase
-
 Thermotoga maritima
2.8.1.B3 ThiI
-
 Thermotoga maritima