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Literature summary extracted from

  • Lamb-Palmer, N.; Singh, M.; Dalton, J.; Singh, J.
    Prokaryotic expression and purification of soluble maize Ac transposase (2013), Mol. Biotechnol., 54, 685-691.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.7.B22 molecular biology the maize activator (Ac) transposase recognizes and excises Ac and Dissociation (Ds) elements and mediates insertion elsewhere in the genome. Insertions of Ds can cause disruption in gene sequences and hence are important functional genomics tool for tagging and cloning of unknown gene sequences Zea mays

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.B22 recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) in soluble form, end-point RT-PCR confirms the integrity of AcTPase mRNA during cell culture, RT-PCR expression analysis Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.B22 nucleus
-
 Zea mays 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.B22 90000
-
-
 Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.B22 Zea mays P08770
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.B22 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography Zea mays

Subunits

EC Number Subunits Comment Organism
2.7.7.B22 ? x * 90000, about, recombinant full-length enzyme, SDS-PAGE Zea mays
2.7.7.B22 More AcTPase is an 807aa protein and consists of three N-terminal nuclear localization signals (residues 44-206), a bipartite DNA binding domain (159-206), a catalytic core domain, and a highly conserved C-terminal dimerization domain (674-754). The catalytic core domain of AcTPase is thought to form a retroviral integrase-like fold Zea mays

Synonyms

EC Number Synonyms Comment Organism
2.7.7.B22 Ac transposase
-
 Zea mays
2.7.7.B22 AcTPase
-
 Zea mays
2.7.7.B22 maize activator transposase
-
 Zea mays

General Information

EC Number General Information Comment Organism
2.7.7.B22 evolution the enzyme belongs to the family of hAT transposases Zea mays
2.7.7.B22 physiological function the maize activator (Ac) transposase recognizes and excises Ac and Dissociation (Ds) elements and mediates insertion elsewhere in the genome. Insertions of Ds can cause disruption in gene sequences, involvement of Ac transposase in Ds movement Zea mays