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Literature summary extracted from

  • Cooley, R.B.; Dubbels, B.L.; Sayavedra-Soto, L.A.; Bottomley, P.J.; Arp, D.J.
    Kinetic characterization of the soluble butane monooxygenase from Thauera butanivorans, formerly Pseudomonas butanovora (2009), Microbiology, 155, 2086-2096.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.230 0.00024
-
butane pH 7.2, 25°C Thauera butanivorans
1.14.13.230 0.00034
-
pentane pH 7.2, 25°C Thauera butanivorans
1.14.13.230 0.00094
-
propane pH 7.2, 25°C Thauera butanivorans
1.14.13.230 0.0022
-
ethane pH 7.2, 25°C Thauera butanivorans
1.14.13.230 1.1
-
methane pH 7.2, 25°C Thauera butanivorans

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.230 Thauera butanivorans Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8 Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively. Q8KQE6 i.e. oxidoreductase BmoC, Q8KQE8 i.e. regulatory protein BmoB
-
1.14.13.230 Thauera butanivorans ATCC 43655 Q8KQF0 and Q8KQE9 and Q8KQE7 and Q8KQE6 and Q8KQE8 Q8KQF0 i.e. alpha-subunit BmoX, Q8KQE9 i.e. beta-subunit BmoY, Q8KQE7 i.e. gamma-subunit BmoZ of hydrolase component, respectively. Q8KQE6 i.e. oxidoreductase BmoC, Q8KQE8 i.e. regulatory protein BmoB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.230 butane + NADH + H+ + O2
-
Thauera butanivorans butan-1-ol + NAD+ + H2O
-
?
1.14.13.230 butane + NADH + H+ + O2
-
Thauera butanivorans ATCC 43655 butan-1-ol + NAD+ + H2O
-
?
1.14.13.230 butane + NADH + H+ + O2
-
Thauera butanivorans butan-2-ol + NAD+ + H2O
-
?
1.14.13.230 butane + NADH + H+ + O2
-
Thauera butanivorans ATCC 43655 butan-2-ol + NAD+ + H2O
-
?
1.14.13.230 ethane + NADH + H+ + O2
-
Thauera butanivorans ethanol + NAD+ + H2O
-
?
1.14.13.230 ethane + NADH + H+ + O2
-
Thauera butanivorans ATCC 43655 ethanol + NAD+ + H2O
-
?
1.14.13.230 methane + NADH + H+ + O2
-
Thauera butanivorans methanol + NAD+ + H2O
-
?
1.14.13.230 methane + NADH + H+ + O2
-
Thauera butanivorans ATCC 43655 methanol + NAD+ + H2O
-
?
1.14.13.230 pentane + NADH + H+ + O2
-
Thauera butanivorans pentan-1-ol + NAD+ + H2O
-
?
1.14.13.230 pentane + NADH + H+ + O2
-
Thauera butanivorans ATCC 43655 pentan-1-ol + NAD+ + H2O
-
?
1.14.13.230 pentane + NADH + H+ + O2
-
Thauera butanivorans pentan-2-ol + NAD+ + H2O
-
?
1.14.13.230 pentane + NADH + H+ + O2
-
Thauera butanivorans pentan-3-ol + NAD+ + H2O
-
?
1.14.13.230 propane + NADH + H+ + O2
-
Thauera butanivorans propan-1-ol + NAD+ + H2O
-
?
1.14.13.230 propane + NADH + H+ + O2
-
Thauera butanivorans propan-2-ol + NAD+ + H2O
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.230 0.008
-
pentane product pentan-3-ol, pH 7.2, 25°C Thauera butanivorans
1.14.13.230 0.039
-
pentane product pentan-2-ol, pH 7.2, 25°C Thauera butanivorans
1.14.13.230 0.12
-
butane product butan-1-ol, pH 7.2, 25°C Thauera butanivorans
1.14.13.230 0.34
-
pentane product pentan-1-ol, pH 7.2, 25°C Thauera butanivorans
1.14.13.230 0.48
-
butane product butan-1-ol, pH 7.2, 25°C Thauera butanivorans
1.14.13.230 0.55
-
propane product propan-1-ol, pH 7.2, 25°C Thauera butanivorans
1.14.13.230 0.76
-
ethane pH 7.2, 25°C Thauera butanivorans
1.14.13.230 0.98
-
propane product propan-2-ol, pH 7.2, 25°C Thauera butanivorans
1.14.13.230 1.3
-
methane pH 7.2, 25°C Thauera butanivorans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.13.230 1.2
-
methane pH 7.2, 25°C Thauera butanivorans
1.14.13.230 350
-
ethane pH 7.2, 25°C Thauera butanivorans
1.14.13.230 690
-
propane overall reaction, pH 7.2, 25°C Thauera butanivorans
1.14.13.230 1200
-
pentane overall reaction, pH 7.2, 25°C Thauera butanivorans
1.14.13.230 2500
-
butane overall reaction, pH 7.2, 25°C Thauera butanivorans