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Literature summary extracted from

  • Chen, W.; Zhang, S.; Jiang, P.; Yao, J.; He, Y.; Chen, L.; Gui, X.; Dong, Z.; Tang, S.Y.
    Design of an ectoine-responsive AraC mutant and its application in metabolic engineering of ectoine biosynthesis (2015), Metab. Eng., 30, 149-155.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.6.1.76 synthesis production of ectoine in Escherichia coli. The Escherichia coli regulatory protein AraC is engineered to recognize ectoine as it snon-natural effector and to activate transcription upon ectoine binding. The ectoine biosynthetic cluster from Halomonas elongata is cloned into Escherichia coli. By engineering the rate-limiting enzyme L-2,4-diaminobutyric acid aminotransferase EctB, ectoine production and the specific activityof the EctB mutant are increased Halomonas elongata

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.76 D180V about 2fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant Halomonas elongata
2.6.1.76 D180V/E36V double mutant displays less activity than mutant D180V Halomonas elongata
2.6.1.76 D180V/F320Y/Q325R about 2.1-3.3fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant Halomonas elongata
2.6.1.76 E36V about 1.5fold increase in ectoine production by strains harboring the ectABC gene cluster containing the mutant Halomonas elongata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.76 0.04
-
 L-2,4-diaminobutanoate mutant D180V/F320Y/Q325R , pH 8.5, 37°C Halomonas elongata
2.6.1.76 0.07
-
 L-2,4-diaminobutanoate mutant D180V, pH 8.5, 37°C Halomonas elongata
2.6.1.76 0.09
-
 L-2,4-diaminobutanoate wild-type, pH 8.5, 37°C Halomonas elongata
2.6.1.76 0.21
-
 2-oxoglutarate mutant D180V/F320Y/Q325R , pH 8.5, 37°C Halomonas elongata
2.6.1.76 0.53
-
 2-oxoglutarate mutant D180V, pH 8.5, 37°C Halomonas elongata
2.6.1.76 1.07
-
 2-oxoglutarate wild-type, pH 8.5, 37°C Halomonas elongata

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.76 Halomonas elongata O52250 CGMCC 1.6329
-
2.6.1.76 Halomonas elongata DSM 2581 O52250 CGMCC 1.6329
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.76 L-2,4-diaminobutanoate + 2-oxoglutarate
-
 Halomonas elongata L-aspartate 4-semialdehyde + L-glutamate
-
 ?
2.6.1.76 L-2,4-diaminobutanoate + 2-oxoglutarate
-
 Halomonas elongata DSM 2581 L-aspartate 4-semialdehyde + L-glutamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.76 EctB
-
 Halomonas elongata

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.76 4.5
-
 2-oxoglutarate wild-type, pH 8.5, 37°C Halomonas elongata
2.6.1.76 8.4
-
 2-oxoglutarate mutant D180V, pH 8.5, 37°C Halomonas elongata
2.6.1.76 25.2
-
 2-oxoglutarate mutant D180V/F320Y/Q325R , pH 8.5, 37°C Halomonas elongata
2.6.1.76 120
-
 L-2,4-diaminobutanoate wild-type, pH 8.5, 37°C Halomonas elongata
2.6.1.76 182
-
 L-2,4-diaminobutanoate mutant D180V, pH 8.5, 37°C Halomonas elongata
2.6.1.76 221
-
 L-2,4-diaminobutanoate mutant D180V/F320Y/Q325R , pH 8.5, 37°C Halomonas elongata

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.76 4
-
 2-oxoglutarate wild-type, pH 8.5, 37°C Halomonas elongata
2.6.1.76 16
-
 2-oxoglutarate mutant D180V, pH 8.5, 37°C Halomonas elongata
2.6.1.76 120
-
 2-oxoglutarate mutant D180V/F320Y/Q325R , pH 8.5, 37°C Halomonas elongata
2.6.1.76 1333
-
 L-2,4-diaminobutanoate wild-type, pH 8.5, 37°C Halomonas elongata
2.6.1.76 2601
-
 L-2,4-diaminobutanoate mutant D180V, pH 8.5, 37°C Halomonas elongata
2.6.1.76 5535
-
 L-2,4-diaminobutanoate mutant D180V/F320Y/Q325R , pH 8.5, 37°C Halomonas elongata