Literature summary extracted from
Lazar, Z.; Dulermo, T.; Neuveglise, C.; Crutz-Le Coq, A.M.; Nicaud, J.M.
Hexokinase - A limiting factor in lipid production from fructose in Yarrowia lipolytica (2014), Metab. Eng., 26, 89-99.
Application
EC Number |
Application |
Comment |
Organism |
---|
2.7.1.1 |
synthesis |
overexpression of HXK1 in a highly modified strain of Yarrowia lipolytica W29 engineered to optimize oil production, combined with Saccharomyces cerevisiae invertase gene expression. For the recombinant strain, sucrose is a better substrate than either of its building blocks, glucose or fructose. Over its 96 h of growth in the bioreactors, this strain produces 9.15g/l of lipids, yielding 0.262g/g of biomass |
Yarrowia lipolytica |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.1.1 |
Yarrowia lipolytica |
O74996 |
- |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.7.1.1 |
Hxk1 |
- |
Yarrowia lipolytica |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
2.7.1.1 |
physiological function |
Overexpression of the HXK1 gene leads to increased hexokinase activity (6.5-12 times higher), a pronounced reduction in cell filamentation in overexpressing strains grown in fructose-based media, and improved biomass production. All overexpressing strains show improved lipid yield and production when grown on fructose, although th eeffect is strain-dependent (23-55% improvement) |
Yarrowia lipolytica |