EC Number | Application | Comment | Organism |
---|---|---|---|
1.14.19.49 | synthesis | a strain lacking CtcP activity accumulates tetracycline with a yield of 18.9 g/l. Overexpressionof ctcP in Streptomyces aureofaciens leads to CTC production to a final titer of 25.9g/l | Kitasatospora aureofaciens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.49 | expression in Escherichia coli | Kitasatospora aureofaciens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.19.49 | 0.056 | - |
(4S)-tetracycline | pH 7.4, 30°C | Kitasatospora aureofaciens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.19.49 | 66300 | - |
- |
Kitasatospora aureofaciens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.49 | (4S)-tetracycline + FADH2 + chloride + O2 + H+ | Kitasatospora aureofaciens | enzyme is specific for (4S)-tetracycline | 7-chloro-(4S)-tetracycline + FAD + 2 H2O | - |
? | |
1.14.19.49 | (4S)-tetracycline + FADH2 + chloride + O2 + H+ | Kitasatospora aureofaciens F3 | enzyme is specific for (4S)-tetracycline | 7-chloro-(4S)-tetracycline + FAD + 2 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.49 | Kitasatospora aureofaciens | S4S3E1 | - |
- |
1.14.19.49 | Kitasatospora aureofaciens F3 | S4S3E1 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.49 | (4S)-tetracycline + FADH2 + chloride + O2 + H+ | enzyme is specific for (4S)-tetracycline | Kitasatospora aureofaciens | 7-chloro-(4S)-tetracycline + FAD + 2 H2O | - |
? | |
1.14.19.49 | (4S)-tetracycline + FADH2 + chloride + O2 + H+ | enzyme is specific for (4S)-tetracycline | Kitasatospora aureofaciens F3 | 7-chloro-(4S)-tetracycline + FAD + 2 H2O | - |
? | |
1.14.19.49 | tetracycline + FADH2 + chloride + O2 + H+ | - |
Kitasatospora aureofaciens | 7-chlorotetracycline + FAD + 2 H2O | - |
? | |
1.14.19.49 | tetracycline + FADH2 + chloride + O2 + H+ | - |
Kitasatospora aureofaciens F3 | 7-chlorotetracycline + FAD + 2 H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.19.49 | ? | x * 66300, SDS-PAGE of recombinant protein with His-tag | Kitasatospora aureofaciens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.19.49 | ctcP | - |
Kitasatospora aureofaciens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.19.49 | 0.0085 | - |
(4S)-tetracycline | pH 7.4, 30°C | Kitasatospora aureofaciens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.19.49 | FADH2 | - |
Kitasatospora aureofaciens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.19.49 | physiological function | chlorination by FADH2-dependent halogenase CtcP is the last inefficient step during chlortetracycline biosynthesis. A strain lacking CtcP activity accumulates tetracycline and is deficient in production of chlortetracycline | Kitasatospora aureofaciens |