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Literature summary extracted from

  • Manhas, R.; Gowri, V.S.; Madhubala, R.
    Leishmania donovani encodes a functional selenocysteinyl-tRNA synthase (2016), J. Biol. Chem., 291, 1203-1220.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.9.1.2 expressed in Escherichia coli SelA-deficient strain JS1 Leishmania donovani

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.9.1.1 cytoplasm
-
 Leishmania donovani 5737
-
2.9.1.2 cytoplasm
-
 Leishmania donovani 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.164 Mg2+ required Leishmania donovani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.164 ATP + L-seryl-tRNASec Leishmania donovani
-
 ADP + O-phospho-L-seryl-tRNASec
-
 ?
2.7.1.164 ATP + L-seryl-tRNASec Leishmania donovani Bob
-
 ADP + O-phospho-L-seryl-tRNASec
-
 ?
2.9.1.1 L-seryl-tRNASec + selenophosphate Leishmania donovani
-
 L-selenocysteinyl-tRNASec + phosphate
-
 ?
2.9.1.1 L-seryl-tRNASec + selenophosphate Leishmania donovani Bob
-
 L-selenocysteinyl-tRNASec + phosphate
-
 ?
2.9.1.2 O-phospho-L-seryl-tRNASec + selenophosphate + H2O Leishmania donovani
-
 L-selenocysteinyl-tRNASec + phosphate
-
 ?
2.9.1.2 O-phospho-L-seryl-tRNASec + selenophosphate + H2O Leishmania donovani Bob
-
 L-selenocysteinyl-tRNASec + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.164 Leishmania donovani
-
 gene PSTK
-
2.9.1.1 Leishmania donovani
-
-
-
2.9.1.2 Leishmania donovani
-
-
-
2.9.1.2 Leishmania donovani Bob
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.9.1.1 promastigote
-
 Leishmania donovani
-
2.9.1.2 promastigote
-
 Leishmania donovani
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.164 ATP + L-seryl-tRNASec
-
 Leishmania donovani ADP + O-phospho-L-seryl-tRNASec
-
 ?
2.7.1.164 ATP + L-seryl-tRNASec
-
 Leishmania donovani Bob ADP + O-phospho-L-seryl-tRNASec
-
 ?
2.9.1.1 L-seryl-tRNASec + selenophosphate
-
 Leishmania donovani L-selenocysteinyl-tRNASec + phosphate
-
 ?
2.9.1.1 L-seryl-tRNASec + selenophosphate
-
 Leishmania donovani Bob L-selenocysteinyl-tRNASec + phosphate
-
 ?
2.9.1.2 O-phospho-L-seryl-tRNASec + selenophosphate + H2O
-
 Leishmania donovani L-selenocysteinyl-tRNASec + phosphate
-
 ?
2.9.1.2 O-phospho-L-seryl-tRNASec + selenophosphate + H2O
-
 Leishmania donovani Bob L-selenocysteinyl-tRNASec + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.9.1.2 homotetramer
-
 Leishmania donovani

Synonyms

EC Number Synonyms Comment Organism
2.7.1.164 O-phosphoseryl-tRNA kinase
-
 Leishmania donovani
2.7.1.164 PSTK
-
 Leishmania donovani
2.9.1.1 SelA
-
 Leishmania donovani
2.9.1.1 Selenocysteinyl-tRNA synthase
-
 Leishmania donovani
2.9.1.2 Selenocysteinyl-tRNA synthase
-
 Leishmania donovani
2.9.1.2 SepSecS
-
 Leishmania donovani

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.164 ATP
-
 Leishmania donovani

General Information

EC Number General Information Comment Organism
2.7.1.164 metabolism the synthesis of selenocysteine, the 21st amino acid, occurs on its transfer RNA, tRNASec. tRNASec is initially aminoacylated with serine by seryl-tRNA synthetase and the resulting seryl moiety is converted to phosphoserine by O-phosphoseryl-tRNA kinase (PSTK) in eukaryotes. The selenium donor, selenophosphate is synthesized from selenide and ATP by selenophosphate synthetase. Selenocysteinyl-tRNA synthase (Sep-SecS) then uses the O-phosphoseryl-tRNASec and selenophosphate to form Sec-tRNASec in eukaryotes. Leishmania SepSecS enzyme is active and able to complement the DELTAselA deletion in Escherichia coli JS1 strain only in the presence of archaeal PSTK, indicating the conserved nature of the PSTK-SepSecS pathway, selenocysteinyl-tRNA synthase is dependent on the action of PSTK enzyme in the Sec insertion pathway Leishmania donovani
2.7.1.164 physiological function the enzyme is essential in the selenocysteine insertion Leishmania donovani
2.9.1.1 physiological function the enzyme is dispensable for the parasite survival Leishmania donovani
2.9.1.2 physiological function the enzyme is dispensable for the parasite survival Leishmania donovani