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Literature summary extracted from

  • Kiesel, S.; Waetzlich, D.; Lange, C.; Reijerse, E.; Broecker, M.J.; Ruediger, W.; Lubitz, W.; Scheer, H.; Moser, J.; Jahn, D.
    Iron-sulfur cluster-dependent catalysis of chlorophyllide a oxidoreductase from Roseobacter denitrificans (2015), J. Biol. Chem., 290, 1141-1154.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.7.15 expressed in Escherichia coli BL21(DE3) cells Roseobacter denitrificans

Protein Variants

EC Number Protein Variants Comment Organism
1.3.7.15 C145A the mutant of subunit BchY is inactive Roseobacter denitrificans
1.3.7.15 C35A the mutant of subunit BchZ is inactive Roseobacter denitrificans
1.3.7.15 C35D the mutant of subunit BchZ is inactive Roseobacter denitrificans
1.3.7.15 C35D the mutant of subunit BchZ shows 60% of wild type activity Roseobacter denitrificans
1.3.7.15 C35D/S94C the mutant of subunit BchZ is inactive Roseobacter denitrificans
1.3.7.15 C35S the mutant of subunit BchZ is inactive Roseobacter denitrificans
1.3.7.15 C62A the mutant of subunit BchY is inactive Roseobacter denitrificans
1.3.7.15 C86A the mutant of subunit BchY shows 50% of wild type activity Roseobacter denitrificans
1.3.7.15 C87A the mutant of subunit BchY is inactive Roseobacter denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.7.15 53000
-
-
 Roseobacter denitrificans
1.3.7.15 56000
-
-
 Roseobacter denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.7.15 bacteriochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate Roseobacter denitrificans
-
 3-acetyl-3-devinylchlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.7.15 Roseobacter denitrificans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.7.15 Ni2+-loaded chelating Sepharose column chromatography Roseobacter denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.7.15 bacteriochlorophyllide a + oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate
-
 Roseobacter denitrificans 3-acetyl-3-devinylchlorophyllide a + reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.7.15 ? x * 53000 + x * 56000 + x * 53000, SDS-PAGE Roseobacter denitrificans

Synonyms

EC Number Synonyms Comment Organism
1.3.7.15 (BchY/BchZ)2 protein
-
 Roseobacter denitrificans
1.3.7.15 BchX subunit Roseobacter denitrificans
1.3.7.15 BchY subunit Roseobacter denitrificans
1.3.7.15 BchZ subunit Roseobacter denitrificans
1.3.7.15 COR
-
 Roseobacter denitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.7.15 [4Fe-4S]-center
-
 Roseobacter denitrificans