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Literature summary extracted from

  • Frank, D.J.; Madrona, Y.; Ortiz de Montellano, P.R.
    Cholesterol ester oxidation by mycobacterial cytochrome P450 (2014), J. Biol. Chem., 289, 30417-30425.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.15.28 in complex with cholesteryl sulfate, sitting drop vapor diffusion method, using 20-25% PEG 3350, 10-13% (v/v) glycerol, and 50 mM MgCl2 Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.15.28 0.0398
-
 cholest-4-en-3-one at pH 7.4 and 25°C Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.28 cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin Mycolicibacterium smegmatis
-
 (25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.28 Mycolicibacterium smegmatis A0R4Q6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.15.28 nickel-nitrilotriacetic acid column chromatography and Q-Sepharose column chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.28 cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin
-
 Mycolicibacterium smegmatis (25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.28 CYP142A2
-
 Mycolicibacterium smegmatis